Genetic diversity in some foreign cassava genotypes using SDS-protein electrophoresis and inter simple sequence repeat markers (ISSRS)‎

Abstract EN

Three foreign genotypes of Cassava were used in this study, American Cassava, Brazilian Cassava and Indonesian Cassava genotypes.

The electrophoretic banding patterns of proteins extracted from the leaves of the three Cassava genotypes.

The results of SDS-PAGE revealed a total number of 15 bands with molecular weights (MW) ranging from about 29 to 220 kDa .

Data revealed 13 common bands (monomorphic), while the remaining 2 band were polymorphic with 13% polymorphism.

The maximum number of bands (15) appeared in Barazilian while the minimum number (13) was present in Indonisian.

Similarity index among the three Cassava genotypes based on protein analysis, carried out using SPSS computer program.

The similarity relationships ranged between 93.1% and 96.6%.

Dendrogram for the genotypes genetic relationships to Cassava genotypes divided into two clusters.

The first cluster contained American and Indonisian , while the second cluster contained Indonisian.

Twelve primers for ISSR were successful in generating reproducible and reliable amplicons for the three forigen Cassava genotypes.

The total number of bands from twelve primers was 100 bands; distributed as 82 polymorphic bands and 18 monomorphic bands.

The nine unique bands were given from interaction among twelve primers ISSR and three Cassava genotypes.

The polymorphism level was differed from primer to another reflecting the primer ability to detect the diversity among Cassava genotypes.

The HB9-primer revealed higher level of polymorphism than the rest of ISSR primers followed by ISSR3 which produced 90% polymorphism.The lowest polymorphism was produced by ISSR2, SH6 with values 66%.

The highest value was similarity between American Cassava and Brazilian Cassava (60.0%) .

The lowest similarity value appeared between American Cassava and Indonisian Cassava (45.9%).