CR technique for detection of giardia lambliain microscopically positive stool samples of the patients in Al-Diwaniyah hospital Iraq
Other Title(s)
استخدام تقنية فحص السلسلة البوليميرية في الكشف عن طفيلي الجيارديا لامبليا في عينات البراز الإيجابية مجهريا المعزولة من المرضى في مستشفى الديوانية العراق
Joint Authors
al-Haydari, Damya makki Hamzah
Malah, Siba Fadil Ali
Source
Journal of al-Qadisiyah for Pure Science
Issue
Vol. 22, Issue 2 (30 Jun. 2017)
Publisher
al-Qadisiyah University College of Science
Publication Date
2017-06-30
Country of Publication
Iraq
Main Subjects
Abstract EN
Giardia lamblia is one of the most common human parasites and causes a lengthily course of nonbacterial diarrhea.
Disease outbreaks due to Giardia infection are often attributed to contaminated food and water supplies.
A major problem associated with detection for this organism is the lack of sensitive and reliable methods.
PCR has the potential to address many of the limitations.
We have performed a PCR-based method for sensitive detection of Giardia .
Because the sensitivity of PCR is a function of the efficiency of DNA extraction from parasite.
Giardia samples were collected from stool of infected persons and first detected microscopically and the second step used of the PCR technique to ensure the infection,DNA was extracted and used of a 619 bp conserved fragment related to the DNA was extracted ,and the GL50803_17190 Eukaryotic peptide chain release factor subunit 1[Giardia lamblia ATCC 50803 ]gene was used as the target for PCR amplification which rarely used.
The results suggest the potential utilities of PCR for sensitive detection of G.Lamblia which isolated from stool of infected persons with Giardiasis.
American Psychological Association (APA)
al-Haydari, Damya makki Hamzah& Malah, Siba Fadil Ali. 2017. CR technique for detection of giardia lambliain microscopically positive stool samples of the patients in Al-Diwaniyah hospital Iraq. Journal of al-Qadisiyah for Pure Science،Vol. 22, no. 2.
https://search.emarefa.net/detail/BIM-850950
Modern Language Association (MLA)
al-Haydari, Damya makki Hamzah& Malah, Siba Fadil Ali. CR technique for detection of giardia lambliain microscopically positive stool samples of the patients in Al-Diwaniyah hospital Iraq. Journal of al-Qadisiyah for Pure Science Vol. 22, no. 2 (2017).
https://search.emarefa.net/detail/BIM-850950
American Medical Association (AMA)
al-Haydari, Damya makki Hamzah& Malah, Siba Fadil Ali. CR technique for detection of giardia lambliain microscopically positive stool samples of the patients in Al-Diwaniyah hospital Iraq. Journal of al-Qadisiyah for Pure Science. 2017. Vol. 22, no. 2.
https://search.emarefa.net/detail/BIM-850950
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-850950