Investigate of the ability of cronobacter Sakazakii isolated from clinical samples of children under two years to induce swimming, swarming and biofilm
Joint Authors
Musa, Muhammad
Zuwayn, Lama Abd al-Hadi
Mutlak, Zariyat Abd al-Rahman
Source
مجلة ابن الهيثم للعلوم الصرفة و التطبيقية
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
English Abstract
The study included 200 samples were collected from children under two years included (50 samples from each of Cerebrospinal fluid, Blood, Stool and Urine) from, Central Children Hospital and Children's Protections Educational Hospital.
Isolates bacterial were obtained cultural, microscopic and biochemical examination and diagnosed to the species by using vitek2 system.
The results showed there were contamination in 6.5% of clinical samples.
The diagnosed colonies which gave pink color on the MacConkey agar, golden yellow color on the Trypton Soy agar and green color on the Birillent Enterobacter sakazakii agar and gave a probability of 99% in the vitek 2 and were identified as Cronobacter sakazakii.
The identification revealed of thirteen isolates: 6(46.16%) isolated from Cerebrospinal fluid samples, 7(53.84%) isolated from blood samples and not isolated bacteria from stool and urine samples.
The results of the investigation of some virulence factors showed that all bacteria isolates were able to swimming with a diameter ranging (1-9 mm) and swarming with a diameter ranging (1-40 mm) and their ability to biofilm formation by using three methods.
The results show the ability of isolates to form biofilm by using Congo red media methods where it is 12 (92.30 %) out of 13 isolated bacteria belonging to C.
sakazakii able to form biofilm on the Congo red media which is 3 (23.07%) were strong production biofilm , 8 (61.53%) were intermediate production biofilm and 1 (7.69% ) were weak biofilm formation , while the 1 (7.69%) unable to form biofilm.
Tubes method were all isolates were able to form biofilm, it were found that 3 (23.07%) isolates strong, and 8 (61.53%) intermediate and 2 ( 15.38%) weak biofilm formation.
Microtiter plate method gave 5 (38.46 %) isolates strong, 6 (46.15%) intermediate and 1 (7.69%) weak biofilm formation.
Data Type
Conference Papers
Record ID
BIM-851542
American Psychological Association (APA)
Zuwayn, Lama Abd al-Hadi& Mutlak, Zariyat Abd al-Rahman& Musa, Muhammad. 2017-12-31. Investigate of the ability of cronobacter Sakazakii isolated from clinical samples of children under two years to induce swimming, swarming and biofilm. International Conference on Biology, Chemistry, Computer Science, Mathematics, and Physics, will take place (2017 : Ibn Al Haitham University of Baghdad, Iraq). . Special issue (2017), pp.123-134.Baghdad Iraq : University of Baghdad College of Education for Pure Science / Ibn al-Haitham.
https://search.emarefa.net/detail/BIM-851542
Modern Language Association (MLA)
Zuwayn, Lama Abd al-Hadi…[et al.]. Investigate of the ability of cronobacter Sakazakii isolated from clinical samples of children under two years to induce swimming, swarming and biofilm. . Baghdad Iraq : University of Baghdad College of Education for Pure Science / Ibn al-Haitham. 2017-12-31.
https://search.emarefa.net/detail/BIM-851542
American Medical Association (AMA)
Zuwayn, Lama Abd al-Hadi& Mutlak, Zariyat Abd al-Rahman& Musa, Muhammad. Investigate of the ability of cronobacter Sakazakii isolated from clinical samples of children under two years to induce swimming, swarming and biofilm. . International Conference on Biology, Chemistry, Computer Science, Mathematics, and Physics, will take place (2017 : Ibn Al Haitham University of Baghdad, Iraq).
https://search.emarefa.net/detail/BIM-851542
