Genetic variation among Saudi tomato (Solanum lycopersicum L.) landraces studied using SDS-PAGE and SRAP markers
Joint Authors
al-Shayi, Najla A.
Sharbaji, Asma
al-Sayigh, Shadha
Dawud, Shadha
al-Anzi, Wala
Miqdadi, Husayn
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 6 (30 Sep. 2018), pp.1007-1015, 9 p.
Publisher
Publication Date
2018-09-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Abstract EN
Genetic diversity among seven Saudi tomato landraces collected from different regions of the country was assessed using SDS-PAGE and molecular (sequence-related amplified polymorphism- SRAP) markers.
A total of 19 alternative protein bands with different mobility rates were identified within a molecular weight range of 9.584–225 KDa, with 53% polymorphism.
Specific protein bands were observed in the ‘‘Hail 548” and ‘‘Qatif 565” landraces.
Genetic similarity based on Jaccard’s coefficient ranged from 0.53 to 1.00, with an average of 0.72.
For molecular evaluation, 143 amplicons (fragments) were generated using 27 SRAP primer pair combinations, of which 88 were polymorphic across all the landraces.
The PIC values ranged from 0.46 to 0.90, with an average of 0.76.
All landraces showed an average of 0.66 similarity coefficient value.
The UPGMA dendrogram supported by principal coordinate analysis (PCoA) revealed clusters of the landraces that almost corresponded to their geographical origin.
Thus, seed storage protein profiling based on SDS-PAGE and SRAP markers can efficiently be used to assess genetic variability among tomato germplasms.
The information obtained in the analysis will be of great interest in the management of ex situ collections for utilization in breeding programs or for direct use in quality markets
American Psychological Association (APA)
al-Shayi, Najla A.& Miqdadi, Husayn& Sharbaji, Asma& al-Sayigh, Shadha& Dawud, Shadha& al-Ghamidi, Salim Safar…[et al.]. 2018. Genetic variation among Saudi tomato (Solanum lycopersicum L.) landraces studied using SDS-PAGE and SRAP markers. Saudi Journal of Biological Sciences،Vol. 25, no. 6, pp.1007-1015.
https://search.emarefa.net/detail/BIM-851786
Modern Language Association (MLA)
al-Ghamidi, Salim Safar…[et al.]. Genetic variation among Saudi tomato (Solanum lycopersicum L.) landraces studied using SDS-PAGE and SRAP markers. Saudi Journal of Biological Sciences Vol. 25, no. 6 (Sep. 2018), pp.1007-1015.
https://search.emarefa.net/detail/BIM-851786
American Medical Association (AMA)
al-Shayi, Najla A.& Miqdadi, Husayn& Sharbaji, Asma& al-Sayigh, Shadha& Dawud, Shadha& al-Ghamidi, Salim Safar…[et al.]. Genetic variation among Saudi tomato (Solanum lycopersicum L.) landraces studied using SDS-PAGE and SRAP markers. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 6, pp.1007-1015.
https://search.emarefa.net/detail/BIM-851786
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1014-1015
Record ID
BIM-851786