Helicobacter pylori in a poultry slaughterhouse : prevalence, genotyping and antibiotic resistance pattern
Joint Authors
Musa, Ihab M.
Hamadah, Muhammad
al-Buhayri, Ayman
Marzuq, Iman
Husayn, Ashgan Muhammad
al-Haji, Jawahir Haji
Heme, Hasan A.
Abidin, Iman
Zahran, Rasha
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 6 (30 Sep. 2018), pp.1072-1078, 7 p.
Publisher
Publication Date
2018-09-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Although Helicobacter pylori (H.
pylori) is a highly significant pathogen, its source remains unclear.
Many people consume chicken daily as a source of animal protein worldwide; thus, hygienic methods of supplying chickens for consumption are critical for public health.
Therefore, our study examined the distribution of the glmM (ureC), babA2, vacA and cagA virulence genes in H.
pylori strains in chicken meat and giblets (gizzards and livers) and the resistance of the strains to various antibiotics.
Ninety chicken meat, gizzard and liver samples were obtained from a semi-automatic abattoir in Sadat City, Egypt, and were cultured and preliminarily analyzed using biochemical tests.
The presence of the ureC, babA2, vacA and cagA genotypes was tested for in samples positive for H.
pylori by multiplex polymerase chain reaction (Multiplex-PCR).
The resistance of H.
pylori to various antimicrobial drugs was tested using the disc diffusion method.
In total, 7 of the 90 chicken samples were positive for H.
pylori (7.78%); in 3/7 (42.85%) samples, the bacteria were found in the chicken liver, while the bacteria were found in the meat in 2/7 (28.57%) and in the gizzard in 2/7 (28.57%) samples.
The total prevalence of both the ureC and babA2 genes in the isolated H.
pylori strains was 100%, while the prevalence of the vacA and cagA genes was 57.1% and 42.9%, respectively.
The resistance of H.
pylori to the antibiotics utilized in our study was 100% for streptomycin; 85.7% for amoxicillin and penicillin; 71.4% for oxytetracycline, nalidixic acid and ampicillin; 57.1% for sulfamethoxazole and erythromycin; and 42.9% for neomycin, chloramphenicol and norfloxacin.
In conclusion, the chicken meat and giblets were tainted by H.
pylori, with a higher occurrence of the ureC, babA2, vacA and cagA genotypes.
Future investigations should investigate the resistance of H.
pylori to various antimicrobial agents in Egypt
American Psychological Association (APA)
Hamadah, Muhammad& al-Buhayri, Ayman& Marzuq, Iman& Musa, Ihab M.& Husayn, Ashgan Muhammad& al-Haji, Jawahir Haji…[et al.]. 2018. Helicobacter pylori in a poultry slaughterhouse : prevalence, genotyping and antibiotic resistance pattern. Saudi Journal of Biological Sciences،Vol. 25, no. 6, pp.1072-1078.
https://search.emarefa.net/detail/BIM-851822
Modern Language Association (MLA)
Hamadah, Muhammad…[et al.]. Helicobacter pylori in a poultry slaughterhouse : prevalence, genotyping and antibiotic resistance pattern. Saudi Journal of Biological Sciences Vol. 25, no. 6 (Sep. 2018), pp.1072-1078.
https://search.emarefa.net/detail/BIM-851822
American Medical Association (AMA)
Hamadah, Muhammad& al-Buhayri, Ayman& Marzuq, Iman& Musa, Ihab M.& Husayn, Ashgan Muhammad& al-Haji, Jawahir Haji…[et al.]. Helicobacter pylori in a poultry slaughterhouse : prevalence, genotyping and antibiotic resistance pattern. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 6, pp.1072-1078.
https://search.emarefa.net/detail/BIM-851822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1077-1078
Record ID
BIM-851822