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1H NMR based metabolite profiling for optimizing the ethanol extraction of Wolfiporia cocos
Joint Authors
Oh, Junsang
Yoon, Deok Hyo
Han, Jae Gu
Choi, Hyung Kyoon
Sung, Gi Ho
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 6 (30 Sep. 2018), pp.1128-1134, 7 p.
Publisher
Publication Date
2018-09-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Abstract EN
Metabolite profiling of Wolfiporia cocos (family: Polyporaceae) had been much advancement in recent days, and its analysis by nuclear magnetic resonance (NMR) spectroscopy has become well established.
However, the highly important trait of W.
cocos still needs advanced protocols despite some standardization.
Partial least squares discriminant analysis (PLS-DA) was used as the multivariate statistical analysis of the 1 H NMR data set.
The PLS-DA model was validated, and the key metabolites contributing to the separation in the score plots of different ethanol W.
cocos extract.
1 H NMR spectroscopy of W.
cocos identified 33 chemically diverse metabolites in D2O, consisting of 13 amino acids, 11 organic acids 2 sugars, 3 sugar alcohols, 1 nucleoside, and 3 others.
Among these metabolites, the levels of tyrosine, proline, methionine, sarcosine, choline, acetoacetate, citrate, 4-aminobutyrate, aspartate, maltose, malate, lysine, xylitol, lactate threonine, leucine, valine, isoleucine, uridine, guanidoacetate, arabitol, mannitol, glucose, and betaine were increased in the 95% ethanol extraction sample compared with the levels in other samples, whereas level of acetate, phenylalanine, alanine, succinate, and fumarate were significantly increased in the 0% ethanol extraction sample.
A biological triterpenoid, namely pachymic acid, was detected from different ethanol P.
cocos extract using 1 H-NMR spectra were found in CDCl3.
This is the first report to perform the metabolomics profiling of different ethanol W.
cocos extract.
These researches suggest that W.
cocos can be used to obtain substantial amounts of bioactive ingredients for use as potential pharmacological and nutraceuticals agents
American Psychological Association (APA)
Oh, Junsang& Yoon, Deok Hyo& Han, Jae Gu& Choi, Hyung Kyoon& Sung, Gi Ho. 2018. 1H NMR based metabolite profiling for optimizing the ethanol extraction of Wolfiporia cocos. Saudi Journal of Biological Sciences،Vol. 25, no. 6, pp.1128-1134.
https://search.emarefa.net/detail/BIM-851855
Modern Language Association (MLA)
Oh, Junsang…[et al.]. 1H NMR based metabolite profiling for optimizing the ethanol extraction of Wolfiporia cocos. Saudi Journal of Biological Sciences Vol. 25, no. 6 (Sep. 2018), pp.1128-1134.
https://search.emarefa.net/detail/BIM-851855
American Medical Association (AMA)
Oh, Junsang& Yoon, Deok Hyo& Han, Jae Gu& Choi, Hyung Kyoon& Sung, Gi Ho. 1H NMR based metabolite profiling for optimizing the ethanol extraction of Wolfiporia cocos. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 6, pp.1128-1134.
https://search.emarefa.net/detail/BIM-851855
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1133-1134
Record ID
BIM-851855