The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion
Joint Authors
Miao, Mingsan
Cao, Lihua
Qiao, Jingyi
Li, Ruiqi
Bai, Ming
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 6 (30 Sep. 2018), pp.1170-1177, 8 p.
Publisher
Publication Date
2018-09-30
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Abstract EN
To investigate the protective mechanism of verbenalin on cerebral ischemia-reperfusion injury.
Middle cerebral artery occlusion in the left hemisphere was induced in rats by filament insertion, and rat model of focal cerebral ischemia-reperfusion was established.
The high, medium and low dose of verbenalin groups were injected in the tail vein of corresponding drugs 10 min before reperfusion, and submitted for 22 h of reperfusion after the operation.
Mortality rate was then calculated, and neurological deficits of rats were scored.
The serum of rats was got to determine the S-100b protein level, and the brain tissue was removed to determine the levels of Bax, Bcl-2, Caspase-3 and ATPase.
TTC staining was performed on the brain tissue to calculate the percentage of cerebral infarct size.
Changes in brain tissue morphology were observed.
Rat model of focal cerebral ischemia-reperfusion was successfully replicated.
In groups that have taken different doses of verbenalin, the mortality rate, neurological deficit score and the percentage of cerebral infarction size were significantly reduced, and the levels of Bax, Caspase-3, S-100b level of the serum in the brain tissue were also significantly reduced.
Increases in the levels of Bcl-2 and ATPase in brain tissue and improvement of pathological damage of hippocampus and cortex were observed.
Verbenalin can inhibit the expression of apoptosis genes, promote the expression of antiapoptosis genes, improve brain microcirculation and energy metabolism, hence reducing cerebral ischemia-reperfusion injury
American Psychological Association (APA)
Cao, Lihua& Miao, Mingsan& Qiao, Jingyi& Bai, Ming& Li, Ruiqi. 2018. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi Journal of Biological Sciences،Vol. 25, no. 6, pp.1170-1177.
https://search.emarefa.net/detail/BIM-851861
Modern Language Association (MLA)
Cao, Lihua…[et al.]. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi Journal of Biological Sciences Vol. 25, no. 6 (Sep. 2018), pp.1170-1177.
https://search.emarefa.net/detail/BIM-851861
American Medical Association (AMA)
Cao, Lihua& Miao, Mingsan& Qiao, Jingyi& Bai, Ming& Li, Ruiqi. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 6, pp.1170-1177.
https://search.emarefa.net/detail/BIM-851861
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1176-1177
Record ID
BIM-851861