The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion

Joint Authors

Miao, Mingsan
Cao, Lihua
Qiao, Jingyi
Li, Ruiqi
Bai, Ming

Source

Saudi Journal of Biological Sciences

Issue

Vol. 25, Issue 6 (30 Sep. 2018), pp.1170-1177, 8 p.

Publisher

Saudi Biological Society

Publication Date

2018-09-30

Country of Publication

Saudi Arabia

No. of Pages

8

Main Subjects

Pharmacology

Abstract EN

To investigate the protective mechanism of verbenalin on cerebral ischemia-reperfusion injury.

Middle cerebral artery occlusion in the left hemisphere was induced in rats by filament insertion, and rat model of focal cerebral ischemia-reperfusion was established.

The high, medium and low dose of verbenalin groups were injected in the tail vein of corresponding drugs 10 min before reperfusion, and submitted for 22 h of reperfusion after the operation.

Mortality rate was then calculated, and neurological deficits of rats were scored.

The serum of rats was got to determine the S-100b protein level, and the brain tissue was removed to determine the levels of Bax, Bcl-2, Caspase-3 and ATPase.

TTC staining was performed on the brain tissue to calculate the percentage of cerebral infarct size.

Changes in brain tissue morphology were observed.

Rat model of focal cerebral ischemia-reperfusion was successfully replicated.

In groups that have taken different doses of verbenalin, the mortality rate, neurological deficit score and the percentage of cerebral infarction size were significantly reduced, and the levels of Bax, Caspase-3, S-100b level of the serum in the brain tissue were also significantly reduced.

Increases in the levels of Bcl-2 and ATPase in brain tissue and improvement of pathological damage of hippocampus and cortex were observed.

Verbenalin can inhibit the expression of apoptosis genes, promote the expression of antiapoptosis genes, improve brain microcirculation and energy metabolism, hence reducing cerebral ischemia-reperfusion injury

American Psychological Association (APA)

Cao, Lihua& Miao, Mingsan& Qiao, Jingyi& Bai, Ming& Li, Ruiqi. 2018. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi Journal of Biological Sciences،Vol. 25, no. 6, pp.1170-1177.
https://search.emarefa.net/detail/BIM-851861

Modern Language Association (MLA)

Cao, Lihua…[et al.]. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi Journal of Biological Sciences Vol. 25, no. 6 (Sep. 2018), pp.1170-1177.
https://search.emarefa.net/detail/BIM-851861

American Medical Association (AMA)

Cao, Lihua& Miao, Mingsan& Qiao, Jingyi& Bai, Ming& Li, Ruiqi. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 6, pp.1170-1177.
https://search.emarefa.net/detail/BIM-851861

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 1176-1177

Record ID

BIM-851861