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In vitro plant regeneration from alginate encapsulated shoot tips of casimairoa edulis L.
Other Title(s)
تجديد نمو القمم النامية المكبسلة معمليا لنبات السابوتا
Author
Abd al-Hadi, Muhammad Rida Abd al-Majid
Source
Egyptian Journal of Desert Research
Issue
Vol. 67, Issue 2 (31 Dec. 2017), pp.315-325, 11 p.
Publisher
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
the present study aimed at developing a method for plant regeneration from alginate-encapsulated shoot tips of Casimairoa edulis.
Shoot tips excised from in vitro proliferated shoots were encapsulated in calcium alginate beads.
The best composition was achieved using 3% sodium alginate and 100 mM calcium chloride (CaCl2.
2H2O).
The survival and regrowth percentages of encapsulation shoot tips decreased gradually with increasing storage duration at 4oC.
Encapsulated shoot tip could be stored at low temperature (4oC) up to 12 weeks with a survival and regrowth frequency of 46.67 and 40.00%, respectively.
Supplementing the alginate matrix with three concentrations of salicylic acid (25, 50 and 75 μM) was found to have pronounced effect on the survival and regrowth percentages.
The survival and regrowth percentage of encapsulated shoot tips with salicylic acid at concentrations of 25, 50 and 75 μM increased to 65, 80 and 60%, respectively after 12 weeks.
The best salicylic acid effect was achieved using the concentration of 50 μM.
The represented synthetic seed technique could be useful in large-scale propagation as well as short-term conservation, germplasm distribution and exchange of C.
edulis.
American Psychological Association (APA)
Abd al-Hadi, Muhammad Rida Abd al-Majid. 2017. In vitro plant regeneration from alginate encapsulated shoot tips of casimairoa edulis L.. Egyptian Journal of Desert Research،Vol. 67, no. 2, pp.315-325.
https://search.emarefa.net/detail/BIM-853378
Modern Language Association (MLA)
Abd al-Hadi, Muhammad Rida Abd al-Majid. In vitro plant regeneration from alginate encapsulated shoot tips of casimairoa edulis L.. Egyptian Journal of Desert Research Vol. 67, no. 2 (2017), pp.315-325.
https://search.emarefa.net/detail/BIM-853378
American Medical Association (AMA)
Abd al-Hadi, Muhammad Rida Abd al-Majid. In vitro plant regeneration from alginate encapsulated shoot tips of casimairoa edulis L.. Egyptian Journal of Desert Research. 2017. Vol. 67, no. 2, pp.315-325.
https://search.emarefa.net/detail/BIM-853378
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 322-324
Record ID
BIM-853378