فعالية المكافحة الحيوية للبكتيريا Bacillus subtilis ضد الفطريات Fusarium oxysporum f. sp. lycopersici, f.solani, Rizhoctonia solani في المختبر و المشتل

Abstract EN

The study was carried out in the Laboratory and Nursery of Biology Department at the University of Aden - Yemen during October - December 2017.

Fusarium oxysporum f.

sp.

lycopersici, F.

solani, Rizhoctonia solani.

The organisms that cause fusarium disease for tomatoes Lycopersicon esculentum Mill.

Bacillus subtilis has been tested as a vital control agent for its effectiveness against these fungi in vitro through inhibition of mycelial growth.

Result of laboratory experiments showed that it was effective against the fungus F.

solani followed by Fusarium oxysporum f.

sp.

lycopersici followed by Rizhoctonia solani with an inhibition ratio of 94.95%, 92.00% and 77.14%, respectively, and significant differences were found at (p = 0.05) compared to the control.

When tested for germination of tomato seeds treated with these bacteria in the greenhouse, it was found that the highest percentage of germination of seeds that fell prey to F.

solani had reached 85% followed by the seeds that were exposed to the fungus Fusarium oxysporum f.

sp.

lycopersici (75%), Rizhoctonia solani (55%), and untreated seeds (20%).

The growth parameters (length of the air segment and length of the roots) showed the treatment of B.

subtilis bacteria against high fungus fungi and found significant statistical differences at 0.05% between the different transactions with each other and the different treatments on one hand and the other side and the highest impact against F.

solani, where the length of the green portion and length of the roots reached 1.45 ± 38.50 mm and 51.5 ± 30.45 mm respectively compared to 1.65 ± 22.35 mm and 1.45 ± 18.35 mm respectively.

This result showed that B.

subtilis bacteria are vital agents that should be harnessed for more biological control applications