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Propagation of sapota (casimiroa edulis) trees by using tissue culture technique
Other Title(s)
إكثار أشجار السبوتا باستخدام تقنية زراعة الأنسجة
Joint Authors
Hassanin, Sabah Anwar
Abu al-Fadl, Rida al-Sayyid al-Sayyid
Abd al-Majid, Muhammad Rida
Source
Egyptian Journal of Desert Research
Issue
Vol. 65, Issue 2 (31 Dec. 2015), pp.257-266, 10 p.
Publisher
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Apromising method for in vitro propagation of white sapota (Casimiroa edulis L.) was established.
Shoot tips and nodal segments of C.
eduils were cultured on Murashige and Skoog (MS) medium supplemented with 2.22 μM 6- benzylamino purine (BAP) and 1.07μM ß-naphthalene acetic acid (NAA) recorded the maximum growth percentage of 88.88 and 77.77 %, and average shoot length of 2.76 and 2.4 cm, for shoot tips and nodal segments, respectively.
Regarding shoots multiplication rate, it was significantly affected by the concentration of BAP, as 6.77 shoots /explant were recorded using MS medium containing 13.32 μM BAP + 4.90 μM N6-2- isopenteny adenine (2iP).
For rooting, half and full strength MS medium supplemented with 0.00, 2.46, 4.90, 9.80, 19.60 and 39.20 μM indol-3-butyric acid (IBA) in combination with 0.00 and 2.69 μM NAA were examined.
The highest rooting percentage, average number of roots/shoot and average root length (44.44 %, 1.88 and 2.46 cm, respectively) were obtained on half strength MS medium containing 19.60 μM IBA and 2.69 μM NAA.
Finally, plantlets were successfully acclimatized to greenhouse conditions and grew vigorously with no apparent phenotype aberrations.
American Psychological Association (APA)
Abd al-Majid, Muhammad Rida& Abu al-Fadl, Rida al-Sayyid al-Sayyid& Hassanin, Sabah Anwar. 2015. Propagation of sapota (casimiroa edulis) trees by using tissue culture technique. Egyptian Journal of Desert Research،Vol. 65, no. 2, pp.257-266.
https://search.emarefa.net/detail/BIM-854592
Modern Language Association (MLA)
Abd al-Majid, Muhammad Rida…[et al.]. Propagation of sapota (casimiroa edulis) trees by using tissue culture technique. Egyptian Journal of Desert Research Vol. 65, no. 2 (2015), pp.257-266.
https://search.emarefa.net/detail/BIM-854592
American Medical Association (AMA)
Abd al-Majid, Muhammad Rida& Abu al-Fadl, Rida al-Sayyid al-Sayyid& Hassanin, Sabah Anwar. Propagation of sapota (casimiroa edulis) trees by using tissue culture technique. Egyptian Journal of Desert Research. 2015. Vol. 65, no. 2, pp.257-266.
https://search.emarefa.net/detail/BIM-854592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 264-265
Record ID
BIM-854592