Changes in protein content of argas (Persicargas)‎ persicus (acari : argasidae)‎ ovary after treatment with allium sativum extract

Abstract EN

Argos (Persicargas) persiais is a parasitic feeder on blood of poultry birds.

After each blood meal a female is capable of producing a large numbers of eggs, the hatched larva remains at-tached to its bird host for seven or more days and draw numerous bulky blood meals.

The ovary is the main factory part for the oogenesis (production of eggs).

The targeting treatment with eco-friendly acaricide is required to disrupt the normal physiology of the ovary.

This study aimed to analyse and quantify' total and fractionated protein in the ovary of A.

persiais females treated with Allium sativum extracts from fresh garlic bulbs.

Group of engorged females were topically treated with garlic extract at 1.44% concentration, and another group with water (control).

All females were crossed independently with normal males.

In order to analyze the proteins of the ovary, untreated females were dissected 0,1,2, 3,4, 5, 6, 7, & 20 days after feeding (DAF).

rep-resented different physiological states of tick and the treated females were dissected 3.4, 5, 6, 7, & 20 DAF.

The ovaries were homogenated and subjected to standard total and fractionated pro-tein techniques.

The protein concentration in ovary wras significantly higher (P<0.05) in controls than in treated ones.

Fractionated protein showed five characteristic bands to vitellogenesis pro- cess in controls.

They reached highest concentration level at 7* days after feeding (DAF).

These five bands disappeared in the treated samples except for one band in the corresponding time.