Modulation of cytokine production from cultured mononuclear cells of leukemia patients by hypericum triquetrifolium turra methanolic extract

Abstract EN

The effect of Hypericum triquetrifolium Turra (Family: Hypericaceae) methanolic extract on an in vitro cytokine release (IL-2, IL-4, IL-10, IL-17A and IFN-c) from cultured mononuclear cells was determined.

The cells were obtained from acute lymphoblastic (ALL) and acute myelogenous leukemic (AML) Iraqi patients.

Two concentrations (1.146 and 1.719 mg/ml) of the extract were tested.

They were correspondent to 20 and 30%, respectively of the plant IC50 (5.73 mg/ml).

Chemical analysis of the extract showed its richness in flavonoids (115.73 mg/ ml).

Assessments of cytokine levels in supernatants of cultured cells revealed that ALL, AML or control cells responded differently to the plant extract in their production of IL-2, IL-10, IL- 17A and IFN-c, but leukemic cells were better than control cells in their response, while there was no effect on IL-4 production.

The results suggested that H.

triquetrifolium methanolic extract exerted immunomodulatory effects on cultured cells.