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The inhibitory effect of trichoderma harzianum ca-07 crude extract against trichophyton mentagrophyte and microsporium canis
Other Title(s)
التأثير المثبط للمستخلص الخام لفطر -07Trichoderma harzianum CA ضد الفطرين Trichophyton mentagrophyte و Microsporium canis المسببة للأمراض الجلدية البشرية
Joint Authors
Asi, Sura Rasan
al-Araji, Ala Muhsin
Source
Issue
Vol. 59, Issue 3B (30 Sep. 2018), pp.1387-1395, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2018-09-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
The study aimed to identify trichoderma harzianum isolates morphologically and by using pcr teqnique and evaluate the antifungal activity of t.
harzianum ca-07 crude extract against trichophyton mentagrophytes and microsporium canis from patients with dermatophytosis.
t.harzianum isolates were collected from the soil of baghdad university gardens and they were identified depending of morphological features on plate and microscopic examination.
the genomic dna of t.harzianum isolate was extracted at a final concentration of (400-600) μg / 2-3 g of wet mycelium and at a purity of 1.6-1.8and dna sample was amplified with each of universal primers (its-1& its-4) to be used for detection of trichoderma species.
the crude extract was extracted from t.harzianum strain ca-07 by ethyl acetate with finalyield of 4.8gm., then different crude extract concentrations (0.5, 1, 2 and4 mg/ml) were used against the clinical pathogenic fungi (t.mentagrophyte and m.canis) using agar well diffusion method.
the results of identification of trichoderma harzianum isolates by using pcr confirmed that the isolate was t.harzianum strain ca-07,and their crude extract exhibited significantly high antifungal activity against t.
mentagrophyte and m.
canis with high growth inhibition zones(14, 12mm) respectively at the lower concentration (0.5mg/ml)of crude extract.
American Psychological Association (APA)
Asi, Sura Rasan& al-Araji, Ala Muhsin. 2018. The inhibitory effect of trichoderma harzianum ca-07 crude extract against trichophyton mentagrophyte and microsporium canis. Iraqi Journal of Science،Vol. 59, no. 3B, pp.1387-1395.
https://search.emarefa.net/detail/BIM-876000
Modern Language Association (MLA)
Asi, Sura Rasan& al-Araji, Ala Muhsin. The inhibitory effect of trichoderma harzianum ca-07 crude extract against trichophyton mentagrophyte and microsporium canis. Iraqi Journal of Science Vol. 59, no. 3B (2018), pp.1387-1395.
https://search.emarefa.net/detail/BIM-876000
American Medical Association (AMA)
Asi, Sura Rasan& al-Araji, Ala Muhsin. The inhibitory effect of trichoderma harzianum ca-07 crude extract against trichophyton mentagrophyte and microsporium canis. Iraqi Journal of Science. 2018. Vol. 59, no. 3B, pp.1387-1395.
https://search.emarefa.net/detail/BIM-876000
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-876000