The effects of extracted peptide from skin of Iraqi frog (rana ridibunda) on human leukemic lymphocytes
Other Title(s)
تأثيرات الببتيدات المستخلصة من جلد الضفدع العراقي Rana ridibunda على الخلايا اللمفاوية لمرضى ابيضاض الدم
Author
Source
Issue
Vol. 16, Issue 1 (31 Mar. 2019), pp.40-48, 9 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2019-03-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
The purified frog skin peptides were tested on leukemic patients lymphocytes, which revealed effects of cytotoxicity.
four frogs (rana ridibunda) were stimulated by single intra-peritoneal injection of norepinephrine-hcl .
five different peptides;1(18) a, 2(19) l, 3(20) i,4(21) e and 5(22) y were isolated and quantified.
the peptide 3(20)i had 5.87% of hemolysis, while healthy human lymphocytes cytotoxic activity was for 2(19)l with inhibition( -10.4%).all peptides were subjected to polyacrylamide gel electrophoresis.
the results revealed peptides 1(18)a, 2(19)l, 3(20)i which appeared as low as 10 kda marker.
theoretically, the whole polypeptide had a molecular weight 7488.61 dalton and contained on 62.405 amino acid (a.a).
the peptide 4(21)e had a highest inhibitory effect(46%) on tumor cell line l20b.
furthermore , peptides effects on acute and chronic myeloid lymphocytic leukemia patients cell cultures revealed peptides selectivity in their action according to their net charge and functional group as reactant proton donor by the evidence of peptide 5(22)y, 16.22 dalton so it was either n—terminus (--nh2) or c—terminus (--oh) that led to cross cell membrane then acted as antigen mediated and activated cells in a high significant value (-142.37± 47.69)for acute myeloid lymphocytic leukemia .both of peptides 3(20) i and 2(19) l were revealed a highly significant differences within chr.40 and chr.
22 of inhibition effects by testing volumes 15 μl and 10 μl .
those inhibition effects were due to peptides reaction with mitochondrial membrane which led to apoptosis .
conclusion; frog skin peptides have a therapeutically worth for malignant diseases.
also some of peptides were activated lymphocytes may to cure immunodeficiency.
American Psychological Association (APA)
al-Khafaji, Ali Jabbar Ulaywi. 2019. The effects of extracted peptide from skin of Iraqi frog (rana ridibunda) on human leukemic lymphocytes. Baghdad Science Journal،Vol. 16, no. 1, pp.40-48.
https://search.emarefa.net/detail/BIM-876195
Modern Language Association (MLA)
al-Khafaji, Ali Jabbar Ulaywi. The effects of extracted peptide from skin of Iraqi frog (rana ridibunda) on human leukemic lymphocytes. Baghdad Science Journal Vol. 16, no. 1 (2019), pp.40-48.
https://search.emarefa.net/detail/BIM-876195
American Medical Association (AMA)
al-Khafaji, Ali Jabbar Ulaywi. The effects of extracted peptide from skin of Iraqi frog (rana ridibunda) on human leukemic lymphocytes. Baghdad Science Journal. 2019. Vol. 16, no. 1, pp.40-48.
https://search.emarefa.net/detail/BIM-876195
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 46-47
Record ID
BIM-876195