Effect of seawater salinity on growth, sodium and potassium concentrations, proline and antioxidant enzymes in tomato callus

Abstract EN

The effect of seawater salinity on callus growth, sodium and potassium concentrations, proline accumulation and some antioxidant enzymes ( ascorbate peroxidase, catalase and superoxide dismutase) activity in the callus of tomato (Lycopersicon esculentum Mill.) cv.

Super Marmmande were investigated.

Seeds of tomato were used as explant and cultured using Pyrex test tubes (150x20 mm) on MS medium with Gamborg vitamins, sucrose, 2,4-D, BA and soldifies with agar, for six weeks, to induce the callus formation and to get a considerable amount of callus to be used in the upcoming experiment.

Seawater was diluted with distilled water and added to the medium at three concentrations (20%, 30% and 40%), 100 mg of the induced callus were cultured on the same previous medium for another six weeks to evaluate the effects of seawater salinity on growth and some biochemical parameters.

Results indicated that high seawater concentration (40%) caused a significant decrease in callus fresh weight and potassium ion concentration, while a significant increase noticed in sodium ion concentration, proline accumulation, and the activity of antioxidant enzymes in the callus