Genetic analysis and detection molecular markers for response to anther culture in wheat (Triticumaestivum L.)‎

Abstract EN

The joint scalling test was employed to study the genetic system controlling anther culture response in wheat; the six generations were derived from the cross between the two highly responsive genotypes ICR-DH and ICR-33 with the nonresponsive genotype Gimmeiza-7.

The results indicated that highly significant differences between the six entries in two crosses for percentage callus formation and number of green plants/100 anthers.

None of the F1’s exceeded its high parent in percentage callus formation.

While, heterosis for the high number of green plants was observed in cross-1.

The additive-dominance model was adequate for percentage callus formation while, the non-allelic gene interaction was found for number of green plants/100 anthers.

Additive gene effects play the major role in controlling the genetic variation in percentage callus formation and number of green plants/100 anthers.

High estimates of the narrow-sense heritability were found in percentage callus formation and number of green plants/100 anthers.The parents in comparison to 4 highly responsive and 4 low responsive plants from each backcross were subjected for RAPD-PCR analyses.A total of 76 DNA bands were generated from all tested genotypes using seven primers.

Only 36.8% of the generated bands were polymorphic while 63.2% were common between the tested genotypes.

Positive andnegative RAPD markers were detected for the genotypes Gimmeiza-7, ICR-DH, and ICR-33.

Three positive molecular markers were detected for anther culture response at 840 bp (OPC-18), 667bp and 462 bp (OPY-5)