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Isolation of endophytic actinomycetes from leaves of tomato plants and their activities against bacterial speck disease
Other Title(s)
عزل الأكتينوميستس الداخلية من أوراق نباتات الطماطم و فاعليتها فى مقاومة مرض التبقع البكتيري
Joint Authors
Hyakumachi, M.
Hasan, Najla
Hasan, Muhammad Ata Allah al-Sayyid
Farraj, Iman Salih
Salam, Muhammad Atif Ahmad
Source
Assuit Journal of Agricultural Sciences
Issue
Vol. 48, Issue 5 (31 Oct. 2017), pp.112-128, 17 p.
Publisher
Assiut University Faculty of Agriculture
Publication Date
2017-10-31
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The biological control agents of endophytic actinomycetes was investigated to determine whether control of bacterial speck of tomato, caused by Pseudomonas syringae pv.
tomato (Pst).
From natural infected tomato leaves showing bacterial speck disease symptoms, six isolates of Pst (Pst1, Pst2, Pst 3, Pst4, Pst5, and Pst DC3000) were isolated, it proved to be pathogenic and able to infect tomato plants under greenhouse condition, causing speck disease symptoms.
From the eighty eight isolates of endophytic actinomycetes recovered from healthy tomato leaves plants, nine isolates were selected and tested for their potential inhibition against bacterial tomato speck disease in vitro and in vivo.
According to16SrDNA analysis the isolates TR-24 and TR-26 of isolated actinomyces proved to be Streptomyces glaucescens.
While the isolates TR-20, TR-32 and TR-34 were identified as Streptomyces graminisoli and isolates TR-25 identified as Streptomyces flavovirens as well as isolates TR-33 and TR-35 were identified as Streptomyces scabies.
Finally isolate TR-36 was identified as Streptomyces roseochromogenus.
Application of nine isolates of Streptomyces by spraying or drenching methods, all Streptomyces isolates were able to reduce disease severity of bacterial speck compared to infected control.
Minutely, the spore suspensions of TR-24 and TR-26 isolates gave the highest reduction of the disease severity.
However, the lowest reduction was achieved by isolate TR-35 compared with other Streptomyces isolates.
American Psychological Association (APA)
Hasan, Najla& Hasan, Muhammad Ata Allah al-Sayyid& Farraj, Iman Salih& Hyakumachi, M.& Salam, Muhammad Atif Ahmad. 2017. Isolation of endophytic actinomycetes from leaves of tomato plants and their activities against bacterial speck disease. Assuit Journal of Agricultural Sciences،Vol. 48, no. 5, pp.112-128.
https://search.emarefa.net/detail/BIM-878923
Modern Language Association (MLA)
Hasan, Najla…[et al.]. Isolation of endophytic actinomycetes from leaves of tomato plants and their activities against bacterial speck disease. Assuit Journal of Agricultural Sciences Vol. 48, no. 5 (2017), pp.112-128.
https://search.emarefa.net/detail/BIM-878923
American Medical Association (AMA)
Hasan, Najla& Hasan, Muhammad Ata Allah al-Sayyid& Farraj, Iman Salih& Hyakumachi, M.& Salam, Muhammad Atif Ahmad. Isolation of endophytic actinomycetes from leaves of tomato plants and their activities against bacterial speck disease. Assuit Journal of Agricultural Sciences. 2017. Vol. 48, no. 5, pp.112-128.
https://search.emarefa.net/detail/BIM-878923
Data Type
Journal Articles
Language
English
Record ID
BIM-878923