Synergistic effect of some plant extracts and antibiotic drugs against staph. aureus isolated from pleural fluid identification of the active compounds

Abstract EN

A total of 75 pyogenic samples were collected from patients examinated for pyogenic infection in sednawy hospital and elmokhtaber laboratories.

s.

aureus isolated from pleural fluid out of five s.

aureus (+ve coagulase, mssa) exhibits resistant against all the concentration of ciprofloxacin 0.5-20, gentamycin 0.5- 5.0 μg/100μl and amikacin 1 μg/100μl.

the highest potent of phytoextracts ei-ther extracted by water or methanol was detected by clove comparing with mint, thyme, sage and garlic, whereas garlic essential oil gave completely abolish of s.

aureus (p.f).

the highest synergism was obtained in combination between the lowest inhibitory concentration of gentamycin 10 μg/100μl or vancomycin 1 μg/100μl or amikacin 2.5 μg/100μl with thyme eso (1:3), which resulted to increase the efficacy by 4.4 or 1.5 or 1.6 respec-tively comparing to recommended dose of the tested antibiotics alone.

increasing the concentra-tion of garlic essential oil (geso) from 10 up to 100 μl/well increment the efficacy of inhibition up to 10, 3.7 and 4.5- fold when standard dose of gentamy-cin, vancomycin and amikacin were used individ-ually against s.

aureus p.f.

the minimum bacteri-cidal concentration of garlic essential oil was rec-orded at 2 μl/ml as it resulted to reduce the count to be 0.04%.

analysis of garlic essential oil by gc-ms dedicated six sulfur compounds represented 88.8644% of total detected compounds in garlic essential oil.