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Radioiodination of cefoperazone with 125I and its biological distribution in mice
Joint Authors
al-Sharawi, Dina Mustafa
al-Rifai, Marwah S.
Khatir, S. I.
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 51, Issue 3 (31 Jul. 2018), pp.62-68, 7 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2018-07-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cefoperazone tracer was radioiodinated with 125I by replacing the hydrogen atom with iodonium cation I+ which is known as an electrophilic substitution reaction.
All the reaction parameters were studied to produce maximum Radiochemical yield (RCY) of 125I-Cefoperazone [125I-Cefo] by using 3.7 MBq of Na125I, 150 µg of cefoperazone as substrate, 100 µg of Chloramine-T as oxidizing agent in water at 60ºC for 20 min, the RCY of 125I-Cefo up to 90.2% The radiochemical yield was determined practically by electrophoresis 0.02M phosphate buffer with pH 7.
A theoretical study was performed using Gaussian 09 to calculate the heat of formation of the expected products.
The tracer was separated and purified by high-pressure liquid chromatography (HPLC) with phosphate buffer pH 6.8: methanol (3:1 v/v).
The biological distribution of the tracer was studied in normal and infected mice with E-coli bacteria and sacrificed after different time’s intervals activity in each organ was counted and expressed as a percentage of the injected activity per organ.
American Psychological Association (APA)
al-Sharawi, Dina Mustafa& al-Rifai, Marwah S.& Khatir, S. I.. 2018. Radioiodination of cefoperazone with 125I and its biological distribution in mice. Arab Journal of Nuclear Sciences and Applications،Vol. 51, no. 3, pp.62-68.
https://search.emarefa.net/detail/BIM-880541
Modern Language Association (MLA)
al-Sharawi, Dina Mustafa…[et al.]. Radioiodination of cefoperazone with 125I and its biological distribution in mice. Arab Journal of Nuclear Sciences and Applications Vol. 51, no. 3 (Jul. 2018), pp.62-68.
https://search.emarefa.net/detail/BIM-880541
American Medical Association (AMA)
al-Sharawi, Dina Mustafa& al-Rifai, Marwah S.& Khatir, S. I.. Radioiodination of cefoperazone with 125I and its biological distribution in mice. Arab Journal of Nuclear Sciences and Applications. 2018. Vol. 51, no. 3, pp.62-68.
https://search.emarefa.net/detail/BIM-880541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 67-68
Record ID
BIM-880541