Genetic diversity in wheat genotypes using random amplified polymorphic DNA (RAPD)
Other Title(s)
التنوع الوراثي في بعض التراكيب الوراثية في القمح بإستخدام التعاظم العشوائي للحمض النووي الديؤكسي الريبوزي (RAPD)
Joint Authors
Muhammad, Mahmud Abu al-Suud al-Rawi
al-Arif, Hamdi Muhammad
Mahmud, Sahar al-Sanusi Abd al-Muti
Taghian, Adil Sayyid
Source
Assuit Journal of Agricultural Sciences
Issue
Vol. 46, Issue 2 (30 Jun. 2015), pp.50-70, 21 p.
Publisher
Assiut University Faculty of Agriculture
Publication Date
2015-06-30
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Abstract EN
Genetic diversity among 75 wheat genotypes (66 hexaploids and 9 tetraploids) was studied using 6 rapd primers.
a total of 76 dna bands were generated by the 6 rapd primers from all genotypes, of which 53 (70.23%) were polymorphic.
the total number of amplification products per primer ranged from 6 (opo06) to 16 (ubc03 and ubc09) with an average of 12.67 bands per primer.
the size of pcr products ranged from 1478bp (opa01) to 91bp(ubc03).
a significant correlation (0.892, p<0.01) was observed between the total number of bands and the number of polymorphic bands.
the six primers amplified 11 unique bands for specific genotypes that would prove to be highly useful in genotype identification and designing the future breeding strategy.
the similarity coefficient values ranged from 0.68 to 1.00, indicating moderate to high genetic similarityin tested loci among the wheat genotypes.
the 75 wheat genotypes were grouped into two major clusters within the dendrogram.
cluster i had the tetraploid genotypes and the hexaploid genotypes were placed in cluster II.
American Psychological Association (APA)
Mahmud, Sahar al-Sanusi Abd al-Muti& Taghian, Adil Sayyid& al-Arif, Hamdi Muhammad& Muhammad, Mahmud Abu al-Suud al-Rawi. 2015. Genetic diversity in wheat genotypes using random amplified polymorphic DNA (RAPD). Assuit Journal of Agricultural Sciences،Vol. 46, no. 2, pp.50-70.
https://search.emarefa.net/detail/BIM-881606
Modern Language Association (MLA)
Mahmud, Sahar al-Sanusi Abd al-Muti…[et al.]. Genetic diversity in wheat genotypes using random amplified polymorphic DNA (RAPD). Assuit Journal of Agricultural Sciences Vol. 46, no. 2 (Jun. 2015), pp.50-70.
https://search.emarefa.net/detail/BIM-881606
American Medical Association (AMA)
Mahmud, Sahar al-Sanusi Abd al-Muti& Taghian, Adil Sayyid& al-Arif, Hamdi Muhammad& Muhammad, Mahmud Abu al-Suud al-Rawi. Genetic diversity in wheat genotypes using random amplified polymorphic DNA (RAPD). Assuit Journal of Agricultural Sciences. 2015. Vol. 46, no. 2, pp.50-70.
https://search.emarefa.net/detail/BIM-881606
Data Type
Journal Articles
Language
English
Record ID
BIM-881606