Early fetal gender determination using convention nested PCR assay of maternal serum : new technologies and clinical applications in Nasiriya Province
Other Title(s)
التحديد المبكر لجنس الجنين بواسطة فحص ال PCR التقليدي المتداخل : تقنيات جديدة و تطبيقات سريرية في محافظة الناصرية
Author
Abd al-Amir, Hibah Abd al-Azim
Source
Issue
Vol. 13, Issue 3 (30 Sep. 2018), pp.81-89, 9 p.
Publisher
University of Thi-Qar Research and Development Department
Publication Date
2018-09-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
This work was carried out in the cancer research unit laboratories, college of medicine, Thi-Qar University, Iraq.
This study demonstrates that a reliable, non-invasive sex determination can be achieved by PCR analysis of maternal serum during the first trimester of pregnancy.
In contrary to the risks of invasive methods that affect both mother and fetus, applying cell-free fetal DNA (cffDNA) is proven highly effective with lower risk.
One of the applications of prenatal diagnosis is fetal gender determination, which is important in fetuses at risk of sex-linked genetic diseases.
In such cases by obtaining the basic information of the gender, necessary time management can be taken in therapeutic to significantly reduce the necessity of applying the invasive methods.
The aim of this study was to use simple multiplex PCR protocol of fetal gender at early stage of gestation applying a multiplex PCR amplification of the ATL1 locus in the FMR1 gene located on the long arm of X-chromosome5 and the Y-chromosome-specific sequence.
A total of 52 pregnant women participated in this study, The ATL1-specific sequences were detected in all 52 serum specimens, while the Y-chromosome-specific fragment were generated in 23 of serum samples.
The Y-specific fragment was not detected in any of the 29 serum samples of serum.
American Psychological Association (APA)
Abd al-Amir, Hibah Abd al-Azim. 2018. Early fetal gender determination using convention nested PCR assay of maternal serum : new technologies and clinical applications in Nasiriya Province. University of Thi-Qar Journal،Vol. 13, no. 3, pp.81-89.
https://search.emarefa.net/detail/BIM-882969
Modern Language Association (MLA)
Abd al-Amir, Hibah Abd al-Azim. Early fetal gender determination using convention nested PCR assay of maternal serum : new technologies and clinical applications in Nasiriya Province. University of Thi-Qar Journal Vol. 13, no. 3 (Sep. 2018), pp.81-89.
https://search.emarefa.net/detail/BIM-882969
American Medical Association (AMA)
Abd al-Amir, Hibah Abd al-Azim. Early fetal gender determination using convention nested PCR assay of maternal serum : new technologies and clinical applications in Nasiriya Province. University of Thi-Qar Journal. 2018. Vol. 13, no. 3, pp.81-89.
https://search.emarefa.net/detail/BIM-882969
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 88-89
Record ID
BIM-882969