التعددية الشكلية لبروتينات التخزين الهوردئين في طرز الشعير المزروعة و البرية

Abstract EN

This research was conducted in the Molecular Biology Laboratory, at the National Commission of Biotechnology (NCBT), Syria during the period 2012 - 2013.SDS-PAGE was used to analyze the genetic diversity in the Hordein polypeptides encoded by multigene families in grains of 33genotypes of cultivated and wild barley collected from different regions.Thirteen alleles at the Hor1 locus with frequencies from 0.83 to14.17%, 17 alleles at the Hor2 locus with frequencies from 0.56 to 13.41% and 7 alleles at the Hor3 locus with frequencies from 3.63 to 30.91% had been recognized.

The number of bands ranged from 8 to 26, depending on the genotype.

The cluster analysis based on Hordein patterns separated the barley genotypes according to their genome types.

The largest cluster included all genotypes, which belonged to the species H.vulgare,H.spontaneumand H.bulbosum.The second cluster included H.murinum and H.marinum genotypes.

results indicated the relevance of the extensive polymorphism in Hordein patterns as a successful tool in identifying the individual genotypes, which can be roeognized according to the genome type.