Molecular cloning and characterization of rosmarinic acid biosynthetic genes and rosmarinic acid accumulation in Ocimum basilicum L.
Joint Authors
Kim, Jae Kwang
Kwon, Do Yeon
Park, Sang Un
Li, Xiaohua
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 3 (31 Mar. 2019), pp.469-472, 4 p.
Publisher
Publication Date
2019-03-31
Country of Publication
Saudi Arabia
No. of Pages
4
Main Subjects
Topics
Abstract EN
We have aimed to investigate the expression of genes related to rosmarinic acid (RA) synthesis and rosmarinic acid content in 2 Ocimum basilicum cultivars, green (cinnamon) and purple (red rubin) basil.
Specifically, genes related to rosmarinic acid biosynthesis were cloned and characterized for O.
basilicum.
We obtained partial cDNAs of tyrosine aminotransferase (TAT) and 4-hydroxyphenylpyruvate reductase (HPPR), which were of 323 bp and 616 bp in size, respectively.
The transcription levels of most genes related to rosmarinic acid synthesis were higher in green basil compared to purple basil, except for ObPAL and Ob4CL in the root.
The highest expression was obtained in the leaves of green basil for all genes and the roots of purple basil for all genes, except for TAT.
The highest rosmarinic acid content was obtained in the leaves of both cultivars, with higher RA accumulating in green basil compared to purple basil.
The leaves had the highest RA content out of all plant organs, with the RA accumulation in the leaves of green basil being 1.64 times higher compared to purple basil.
Further study is required to investigate whether a similar trend is observed across O.
basilicum cultivars of different color types
American Psychological Association (APA)
Kwon, Do Yeon& Li, Xiaohua& Kim, Jae Kwang& Park, Sang Un. 2019. Molecular cloning and characterization of rosmarinic acid biosynthetic genes and rosmarinic acid accumulation in Ocimum basilicum L.. Saudi Journal of Biological Sciences،Vol. 26, no. 3, pp.469-472.
https://search.emarefa.net/detail/BIM-887226
Modern Language Association (MLA)
Kwon, Do Yeon…[et al.]. Molecular cloning and characterization of rosmarinic acid biosynthetic genes and rosmarinic acid accumulation in Ocimum basilicum L.. Saudi Journal of Biological Sciences Vol. 26, no. 3 (Mar. 2019), pp.469-472.
https://search.emarefa.net/detail/BIM-887226
American Medical Association (AMA)
Kwon, Do Yeon& Li, Xiaohua& Kim, Jae Kwang& Park, Sang Un. Molecular cloning and characterization of rosmarinic acid biosynthetic genes and rosmarinic acid accumulation in Ocimum basilicum L.. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 3, pp.469-472.
https://search.emarefa.net/detail/BIM-887226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 472
Record ID
BIM-887226