The research on the mechanism of tsoong inhibiting for colon cancer
Joint Authors
Cao, Yong
Dai, Fei
Luan, Yunpeng
Zhao, Youjie
Li, Yanmei
Jia, Lukun
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 3 (31 Mar. 2019), pp.605-613, 9 p.
Publisher
Publication Date
2019-03-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
The roots of Codonopis bulleynana Forest ex diels (cbFed), locally known as Tsoong, have been used as a tonic food.
Tsoong has wide range of pharmacological effects, including anticancer effects.
In the present study, the anticancer activity of Tsoong and its potential molecular mechanisms were investigated.
Using high throughput sequencing the apoptotic pathway was ranked as one of the most important pathways and the differential expressions of apoptosis-related genes such as Casp3, Casp6 and Apaf1 were identified.
The following experiments were qRT-PCR which were used to verify the genes.
In vitro, cell counting kit-8 (CCK-8) assays and flow cytometry in HCT116 and SW480 colon cancer cell were used to assess the anti-proliferation and apoptosis-promoting activities of Tsoong.
In vivo, the antitumor effect of Tsoong was assessed in colon cancer-bearing nude mice as a xenograft model.
H&E staining was performed with oxaliplatin set as a positive control.
The results showed that Tsoong up-regulated apoptosis-related genes, inhibited tumor cell proliferation, promoted tumor cellapoptosis in a dose-dependent manner and restrained the growth of colon neoplasm.
The effects of a high dose of Tsoong on colon cancer cells were similar to those of oxaliplatin.
Our results may ultimately help in the development of diagnostic and therapeutic strategies to control this devastating disease.
Therefore, Tsoong may be a promising Chinese herbal compound for development for use in cancer therapy.
American Psychological Association (APA)
Cao, Yong& Dai, Fei& Li, Yanmei& Jia, Lukun& Luan, Yunpeng& Zhao, Youjie. 2019. The research on the mechanism of tsoong inhibiting for colon cancer. Saudi Journal of Biological Sciences،Vol. 26, no. 3, pp.605-613.
https://search.emarefa.net/detail/BIM-887245
Modern Language Association (MLA)
Cao, Yong…[et al.]. The research on the mechanism of tsoong inhibiting for colon cancer. Saudi Journal of Biological Sciences Vol. 26, no. 3 (Mar. 2019), pp.605-613.
https://search.emarefa.net/detail/BIM-887245
American Medical Association (AMA)
Cao, Yong& Dai, Fei& Li, Yanmei& Jia, Lukun& Luan, Yunpeng& Zhao, Youjie. The research on the mechanism of tsoong inhibiting for colon cancer. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 3, pp.605-613.
https://search.emarefa.net/detail/BIM-887245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 613
Record ID
BIM-887245