Evaluation of staining methods for diagnosis of trichomoniasis in clinically suspected women in Jazan, KSA

Abstract EN

Forty female patients experiencing symptoms suggestive of trichomoniasis were included into the present investigation.

Patients` self-obtained vaginal swabs were gathered and examined for T.

vaginalis by wet mount, Giemsa stain, Gram stain and culture techniques.

T.

vaginalis was recognized in 11 out of 40 specimens by culture (27.5 %), 10 by wet mount (25 %), 8 by Gram stain (20 %) and 7 by Giemsa stain (17.5 %).

Contrasted with culture as the standard technique, Giemsa staining method indicated 63.6 % sensitivity compared with 54.5% for wet mount or Gram staining.

Giemsa staining specificity was 100 % contrasted with 86.2 % for wet mount and 93.1 % for Gram stain.

In addition, a considerable agreement was found amongst culture and Giemsa staining (Kappa= 0.717) while moderate agreement of Gram stain (Kappa= 0.52) and wet mount (Kappa = 0.419) with culture was found.