Immuno-magnetic beads elisa for diagnosis of schistosoma mansoni infection
Joint Authors
Miabid, Iman Mustafa H.
Hasan, Isam A.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 49, Issue 1 (30 Apr. 2019), pp.51-59, 9 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2019-04-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Topics
Abstract EN
Diagnosis of Schistosoma mansoni infection is a challenge in low transmission regions.
Serological diagnosis proved more sensitive and extensively used to detect anti- Schistosoma antibodies.
This study evaluated the immunomagnetic beads-enzyme-linked immunosorbent assay (IMB-ELISA) for diagnosing S.
mansoni infection in comparison with the commercial IHA, indirect ELISA and microscopy stool examination.
A total of 100 subjects were divided into four groups (25 each).
G1: cases actively passing S.
mansoni eggs, G2: chronic cases of S.
mansoni, G3: cases with other parasitosis and G4: normal healthy subjects.
S.
mansoni diagnostic rate was 25%, 46%, 48%, & 50% by parasitological, IHA, Indirect ELISA & IMB-ELISA, respectively.
In G1, the four tests gave positivity rates of 100%, 88%, 96%, & 100%, respectively.
In G2, the four tests gave 0%, 84%, 88% & 96%, respectively.
Sensitivity of various tests were estimated in relation to total true positive S.
mansoni cases (G1 & G2), and gave 50%, 86%, 92% & 98% for the applied tests, respectively.
Cross-reactivity rates were estimated in relation to S.
mansoni negative cases (G3 & G4).
Specificity rates were 100%, 94%, 96% & 98%, respectively
American Psychological Association (APA)
Miabid, Iman Mustafa H.& Hasan, Isam A.. 2019. Immuno-magnetic beads elisa for diagnosis of schistosoma mansoni infection. Journal of the Egyptian Society of Parasitology،Vol. 49, no. 1, pp.51-59.
https://search.emarefa.net/detail/BIM-888981
Modern Language Association (MLA)
Miabid, Iman Mustafa H.& Hasan, Isam A.. Immuno-magnetic beads elisa for diagnosis of schistosoma mansoni infection. Journal of the Egyptian Society of Parasitology Vol. 49, no. 1 (Apr. 2019), pp.51-59.
https://search.emarefa.net/detail/BIM-888981
American Medical Association (AMA)
Miabid, Iman Mustafa H.& Hasan, Isam A.. Immuno-magnetic beads elisa for diagnosis of schistosoma mansoni infection. Journal of the Egyptian Society of Parasitology. 2019. Vol. 49, no. 1, pp.51-59.
https://search.emarefa.net/detail/BIM-888981
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 59
Record ID
BIM-888981