Immuno-magnetic beads elisa for diagnosis of schistosoma mansoni infection

Abstract EN

Diagnosis of Schistosoma mansoni infection is a challenge in low transmission regions.

Serological diagnosis proved more sensitive and extensively used to detect anti- Schistosoma antibodies.

This study evaluated the immunomagnetic beads-enzyme-linked immunosorbent assay (IMB-ELISA) for diagnosing S.

mansoni infection in comparison with the commercial IHA, indirect ELISA and microscopy stool examination.

A total of 100 subjects were divided into four groups (25 each).

G1: cases actively passing S.

mansoni eggs, G2: chronic cases of S.

mansoni, G3: cases with other parasitosis and G4: normal healthy subjects.

S.

mansoni diagnostic rate was 25%, 46%, 48%, & 50% by parasitological, IHA, Indirect ELISA & IMB-ELISA, respectively.

In G1, the four tests gave positivity rates of 100%, 88%, 96%, & 100%, respectively.

In G2, the four tests gave 0%, 84%, 88% & 96%, respectively.

Sensitivity of various tests were estimated in relation to total true positive S.

mansoni cases (G1 & G2), and gave 50%, 86%, 92% & 98% for the applied tests, respectively.

Cross-reactivity rates were estimated in relation to S.

mansoni negative cases (G3 & G4).

Specificity rates were 100%, 94%, 96% & 98%, respectively