New approach for determination of specific trematoda snail intermediate hosts
Joint Authors
al-Bahi, Muhammad M.
Kamil, Nansi O.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 49, Issue 1 (30 Apr. 2019), pp.99-104, 6 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2019-04-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The compatible miracidium is able to inhibit without stimulate the immune defense mechanism of its snail.
So, snail failed to recognize it as foreign body.
A level of antigenic similarity must be present between the miracidia and its natural snail intermediate host (IH).
The present study investigated the level of compatibility or difference in molecular structures between miracidia and their specific snails IH using protein fractionation and western blot (EITB).
Specific anti-snail rat hyper immune sera (RHIS) were obtained by rat vaccination.
This RHIS was reacted versus different miracidial antigens (Ag) to determine the compatibility level between any miracidium and the target snail Ag using EITB.
The results showed five polypeptides bands at molecular weight (MW) of 100kda, 60kda, 50kda, 45kda & 35-38kda, common between S.
mansoni miracidium Ag and B.
alexandrina feet Ag.
Three fractions at MW of 58kda, 38-42kda & 22-24kda were recognized in fractionated F.
gigantica miracidium versus anti-L.
natalensis Ab.
Six polypeptide bands at MW of 48-50kda, 38-40kda, 30kda, 25 kda, 22.5kda & 15kda were recognized between E.
revolutum miracidia and P.
acuta fractionated feet Ag.
There was no polypeptide bands between any miracidium and snail other than its specific one
American Psychological Association (APA)
al-Bahi, Muhammad M.& Kamil, Nansi O.. 2019. New approach for determination of specific trematoda snail intermediate hosts. Journal of the Egyptian Society of Parasitology،Vol. 49, no. 1, pp.99-104.
https://search.emarefa.net/detail/BIM-888989
Modern Language Association (MLA)
al-Bahi, Muhammad M.& Kamil, Nansi O.. New approach for determination of specific trematoda snail intermediate hosts. Journal of the Egyptian Society of Parasitology Vol. 49, no. 1 (Apr. 2019), pp.99-104.
https://search.emarefa.net/detail/BIM-888989
American Medical Association (AMA)
al-Bahi, Muhammad M.& Kamil, Nansi O.. New approach for determination of specific trematoda snail intermediate hosts. Journal of the Egyptian Society of Parasitology. 2019. Vol. 49, no. 1, pp.99-104.
https://search.emarefa.net/detail/BIM-888989
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 104
Record ID
BIM-888989