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Evaluations of Alkyl hydroperoxide reductase B cell antigen epitope as a potential epitope vaccine against Campylobacter jejuni
Joint Authors
Lou, Hongqiang
Li, Xusheng
Guo, Fangming
Ding, Mingxing
Hu, Ye
Chen, Haohao
Yan, Jie
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 6 (30 Sep. 2019), pp.1117-1122, 6 p.
Publisher
Publication Date
2019-09-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Objective: The present study aimed to screen and find alkyl hydroperoxide reductase (AhpC) B cell dominant epitope of Campylobacter jejuni (C.
jejuni).
Materials and methods: Bio-informatic algorithms were used to predict B cell epitopes of AhpC.
The AhpC protein and chemically synthesized antigenic epitopes of C.
jejuni were considered as antigens, and the AhpC antibody was used as the primary antibody, ELISA and dot blot were used to analyze and screen the dominant epitope.
The specific IgG of mice serum and IL-4 in splenocyte culture supernatant were detected by ELISA.
The protective efficacy was evaluated by animal disease index and tissue histopathological staining of the jejunum.
Results: Seven epitopes of AhpC were predicted, one epitope (AhpC4–16) was found to recognize the antibodies of AhpC and had strong antigenicity by ELISA and dot blot analysis.
In epitope AhpC4–16 immunized mice, specific IgG of serum and IL-4 in splenocyte culture supernatant were significantly higher.
The illness index decreased significantly, the protective rate was 66.67%.
Histopathology displayed that the jejunum morphology was better than the control group.
Conclusions: These findings suggested that epitope AhpC4–16 showed effective protective role against C.
jejuni and is a candidate epitope of vaccine against this pathogen
American Psychological Association (APA)
Lou, Hongqiang& Li, Xusheng& Guo, Fangming& Ding, Mingxing& Hu, Ye& Chen, Haohao…[et al.]. 2019. Evaluations of Alkyl hydroperoxide reductase B cell antigen epitope as a potential epitope vaccine against Campylobacter jejuni. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1117-1122.
https://search.emarefa.net/detail/BIM-889183
Modern Language Association (MLA)
Lou, Hongqiang…[et al.]. Evaluations of Alkyl hydroperoxide reductase B cell antigen epitope as a potential epitope vaccine against Campylobacter jejuni. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1117-1122.
https://search.emarefa.net/detail/BIM-889183
American Medical Association (AMA)
Lou, Hongqiang& Li, Xusheng& Guo, Fangming& Ding, Mingxing& Hu, Ye& Chen, Haohao…[et al.]. Evaluations of Alkyl hydroperoxide reductase B cell antigen epitope as a potential epitope vaccine against Campylobacter jejuni. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1117-1122.
https://search.emarefa.net/detail/BIM-889183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1122
Record ID
BIM-889183