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TRPV4 plays an important role in rat prefrontal cortex changes induced by acute hypoxic exercise
Joint Authors
Li, Yan
Han, Peng
Hu, Yanxin
Huang, Xing
Gu, Boya
Zhu, Rongxin
Han, Tianyu
Yu, Jiabei
Mu, Lianwei
Li, Cui
Zhang, Weijia
Yang, Yun
Hu, Yang
Zhao, Li
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 6 (30 Sep. 2019), pp.1194-1206, 13 p.
Publisher
Publication Date
2019-09-30
Country of Publication
Saudi Arabia
No. of Pages
13
Main Subjects
Topics
Abstract EN
Objective This study aims to investigate the effects of TRPV4 on acute hypoxic exercise-induced central fatigue, in order to explore the mechanism in central for exercise capacity decline of athletes in the early stage of altitude training.
Methods 120 male Wistar rats were randomly divided into 12 groups: 4 normoxia groups (quiet group, 5-level group, 8-level group, exhausted group), 4 groups at simulated 2500 m altitude (grouping as before), 4 groups at simulated 4500 m altitude (grouping as before), 10 in each group.
With incremental load movement, materials were drawn corresponding to the load.
Intracellular calcium ion concentration was measured by HE staining, enzyme-linked immunosorbent assay, immunohistochemistry, RT-qPCR, Fluo-4/AM and Fura-2/AM fluorescence staining.
Results (1) Hypoxic 2–5 groups showed obvious venous congestion, with symptoms similar to normoxia-8 group; Hypoxic 2–8 groups showed meningeal loosening edema, infra-meningeal venous congestion, with symptoms similar to normoxia-exhausted group and hypoxic 1-exhaused group.
(2) For 5,6-EET, regardless of normoxic or hypoxic environment, significant or very significant differences existed between each exercise load group (normoxic − 5 level 20.58 ± 0.66 pg/mL, normoxic − 8 level 23.15 ± 0.46 pg/mL, normoxic - exhausted 26.66 ± 0.71 pg/mL; hypoxic1-5 level 21.72 ± 0.43 pg/mL, hypoxic1-8 level 24.73 ± 0.69 pg/mL, hypoxic 1-exhausted 28.68 ± 0.48 pg/mL; hypoxic2-5 level 22.75 ± 0.20 pg/mL, hypoxic2-8 level 25.62 ± 0.39 pg/mL, hypoxic 2-exhausted 31.03 ± 0.41 pg/mL) and quiet group in the same environment(normoxic-quiet 18.12 ± 0.65 pg/mL, hypoxic 1-quiet 19.94 ± 0.43 pg/mL, hypoxic 2-quiet 21.72 ± 0.50 pg/mL).
The 5,6-EET level was significantly or extremely significantly increased in hypoxic 1 environment and hypoxic 2 environment compared with normoxic environment under the same load.
(3) With the increase of exercise load, expression of TRPV4 in the rat prefrontal cortex was significantly increased; hypoxic exercise groups showed significantly higher TRPV4 expression than the normoxic group.
(4) Calcium ion concentration results showed that in the three environments, 8 level group (normoxic-8 190.93 ± 6.11 nmol/L, hypoxic1-8 208.92 ± 6.20 nmol/L, hypoxic2-8 219.13 ± 4.57 nmol/L) showed very significant higher concentration compared to quiet state in the same environment (normoxic-quiet 107.11 ± 0.49 nmol/L, hypoxic 1-quiet 128.48 ± 1.51 nmol/L, hypoxic 2-quiet 171.71 ± 0.84 nmol/L), and the exhausted group in the same environment (normoxic-exhausted 172.51 ± 3.30 nmol/L, hypoxic 1-exhausted 164.54 ± 6.01 nmol/L, hypoxic 2-exhausted 154.52 ± 1.80 nmol/L) had significant lower concentration than 8-level group; hypoxic2-8 had significant higher concentration than normoxic-8.
American Psychological Association (APA)
Huang, Xing& Hu, Yanxin& Zhao, Li& Gu, Boya& Zhu, Rongxin& Li, Yan…[et al.]. 2019. TRPV4 plays an important role in rat prefrontal cortex changes induced by acute hypoxic exercise. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1194-1206.
https://search.emarefa.net/detail/BIM-889194
Modern Language Association (MLA)
Huang, Xing…[et al.]. TRPV4 plays an important role in rat prefrontal cortex changes induced by acute hypoxic exercise. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1194-1206.
https://search.emarefa.net/detail/BIM-889194
American Medical Association (AMA)
Huang, Xing& Hu, Yanxin& Zhao, Li& Gu, Boya& Zhu, Rongxin& Li, Yan…[et al.]. TRPV4 plays an important role in rat prefrontal cortex changes induced by acute hypoxic exercise. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1194-1206.
https://search.emarefa.net/detail/BIM-889194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1206
Record ID
BIM-889194