Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia
Author
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 6 (30 Sep. 2019), pp.1247-1252, 6 p.
Publisher
Publication Date
2019-09-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Accumulation of petroleum hydrocarbon residual considered a major environmental problem in the kingdom of Saudi Arabia cause of intensive efforts for oil detecting.
Until now, In situ biodegradation considered the most effective method for petroleum hydrocarbon residual biodegradation.
The aim of this study is isolation and identification biodegradable capability bacteria from contaminated sites in Khurais oil field, Dhahran, Saud Arabia via Different morphological and biochemical and molecular methods.
Furthermore, degradation level in contaminated liquid medium and soil were evaluated.
Three bacterial strains were selected from petroleum-contaminated soils of Khurais oil field depending on their capacity to grow in the existence of hydrocarbon components and identified according to morphological, biochemical.
Interestingly, 16S rDNA sequencing fingerprinting results confirmed our bacterial identification as Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereu.
Phyllogenetic tree was constructed and genetic similarity was calculated according to alignments results.
Biodegradation patterns for different three isolates were reflected varied degradation ability for three isolates regarding incubation time.
Different features were studied for three biodegrading bacterial strains and identified as Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereus.
Remarkable degradation rate % patterns for hydrocarbons residual were recorded for all three isolates with varied.
American Psychological Association (APA)
al-Ḍaban, Fahad A.. 2019. Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1247-1252.
https://search.emarefa.net/detail/BIM-889199
Modern Language Association (MLA)
al-Ḍaban, Fahad A.. Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1247-1252.
https://search.emarefa.net/detail/BIM-889199
American Medical Association (AMA)
al-Ḍaban, Fahad A.. Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1247-1252.
https://search.emarefa.net/detail/BIM-889199
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1252
Record ID
BIM-889199