Effect of D-mannose on gene expression of neuraminidase produced from different clinical isolates of pseudomonas aeruginosa

Other Title(s)

تاثير دي-مانوز على التعبير الجيني للنيورامينيديز المنتج من بعض العزلات السريرية لبكتريا Pseudomonas aeruginosa

Joint Authors

al-Rabii, Baha Abd Allah Laftah
Shihab, Zaynah Hashim

Source

Baghdad Science Journal

Issue

Vol. 16, Issue 2 (30 Jun. 2019), pp.291-298, 8 p.

Publisher

University of Baghdad College of Science for Women

Publication Date

2019-06-30

Country of Publication

Iraq

No. of Pages

8

Main Subjects

Biology

Topics

Abstract EN

The present work aimed to investigate the neuraminidase (nan1) gene expression in 32 different clinical isolates of Pseudomonas aeruginosa to explore the role of the enzyme in different types of infection and might give a better understanding of host cell-pathogens interaction.

In addition, the effect of monosaccharide D-mannose on neuraminidase gene expression in eight isolates was studied by utilizing a reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

The results demonstrated that the highest expression of nan1 gene was in otitis samples (208,913.81) which were significantly higher than that from other infections (P < 0.01).

While, the concentrations of gene copies obtained from urine, sputum and burns samples were 93,535.34, 92,254.64 and 74,029.63respectively.

While the least expression in wound samples (32,017.06).

This suggests that neuraminidase in ear samples might be more virulent and invasive followed by that from urine, sputum, burns and wounds samples.

The considerable interest of addition D-mannose significantly reduced the rate of neuraminidase activity reached fivefold in some isolates.

This indicates that D-mannose down regulates nan1 gene expression.

Hence, this sugar could be used in the development of potential new antibacterial agents where it acts as a competitive neuraminidase inhibitors.

American Psychological Association (APA)

Shihab, Zaynah Hashim& al-Rabii, Baha Abd Allah Laftah. 2019. Effect of D-mannose on gene expression of neuraminidase produced from different clinical isolates of pseudomonas aeruginosa. Baghdad Science Journal،Vol. 16, no. 2, pp.291-298.
https://search.emarefa.net/detail/BIM-890856

Modern Language Association (MLA)

Shihab, Zaynah Hashim& al-Rabii, Baha Abd Allah Laftah. Effect of D-mannose on gene expression of neuraminidase produced from different clinical isolates of pseudomonas aeruginosa. Baghdad Science Journal Vol. 16, no. 2 (2019), pp.291-298.
https://search.emarefa.net/detail/BIM-890856

American Medical Association (AMA)

Shihab, Zaynah Hashim& al-Rabii, Baha Abd Allah Laftah. Effect of D-mannose on gene expression of neuraminidase produced from different clinical isolates of pseudomonas aeruginosa. Baghdad Science Journal. 2019. Vol. 16, no. 2, pp.291-298.
https://search.emarefa.net/detail/BIM-890856

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 296-297

Record ID

BIM-890856