Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG
Joint Authors
Miao, Mingsan
Yan, Xiaoli
Wei, Zhenzhen
Peng, Mengfan
Zhu, Zhengwang
Li, Mengyan
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 4 (31 May. 2019), pp.821-827, 7 p.
Publisher
Publication Date
2019-05-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Aims : Explore the effects of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by dehydroepiandrosterone (DHEA) combined human chorionic gonadotropin (HCG).
Methods : Except the blank group, the rest of the rats were injected with DHEA 6 mg/100 g on the back of the neck and 1.5 IU HCG each day, for 21 consecutive days.
On the 16th day of modeling, vaginal smear was performed to select the model rats, which were randomly divided into model group, dacin-35 group, large, middle and small dose dodder total flavonoids groups, and given the medicine for three weeks.
At the end of the last administration, take samples, so as to calculate the ovaries and uterus indexes, measure serum LH / FSH ratio, P, PRL and INS levels, fixed the uterus and pancreas in 10% formalin solution and stained with HE to observe the morphological changes of the organs.
And measure the expression of TNFa and IGF-l proteins in ovaries by immunohistochemistry.
Results : Compared with the blank group, ovarian and uterine indexes, serum LH/FSH ratio, serum PRL and INS levels, ovary TNF-a and IGF-l protein expression were significantly increased, and significant pathological changes were observed in the uterine and pancreatic tissues in model group (P < 0.01).
While the serum P level decreased significantly (P < 0.01), Compared with the model group, the ovarian and uterine indexes, serum LH/FSH ratio, serum P, PRL and INS levels, ovary TNF-a protein expression were significantly decreased in large, middle and small dose dodder total flavonoids groups (P < 0.01); The expression of IGF-1 protein was decreased and uterus pathological changes were improved in different extents (P < 0.01 or P < 0.05), pancreas pathological changes were improved significantly (P < 0.01).
Conclusion: PCOS rat models was successfully replicated.
Dodder total flavone can protect PCOS rats induced by DHEA combined HCG by different action pathways.
American Psychological Association (APA)
Miao, Mingsan& Peng, Mengfan& Zhu, Zhengwang& Yan, Xiaoli& Wei, Zhenzhen& Li, Mengyan. 2019. Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG. Saudi Journal of Biological Sciences،Vol. 26, no. 4, pp.821-827.
https://search.emarefa.net/detail/BIM-891161
Modern Language Association (MLA)
Miao, Mingsan…[et al.]. Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG. Saudi Journal of Biological Sciences Vol. 26, no. 4 (May. 2019), pp.821-827.
https://search.emarefa.net/detail/BIM-891161
American Medical Association (AMA)
Miao, Mingsan& Peng, Mengfan& Zhu, Zhengwang& Yan, Xiaoli& Wei, Zhenzhen& Li, Mengyan. Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 4, pp.821-827.
https://search.emarefa.net/detail/BIM-891161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 826-827
Record ID
BIM-891161