Engineered Ssp DnaX inteins for protein splicing with flanking proline residues

Abstract EN

Inteins are internal protein sequences capable of catalyzing a protein splicing reaction by self-excising from a precursor protein and simultaneously joining the flanking sequences with a peptide bond.

Split inteins have separate pieces (N-intein and C-intein) that reassemble non-covalently to catalyze a protein trans-splicing reaction joining two polypeptides.

Protein splicing has become increasingly useful tools in many fields of biological research and biotechnology.

However, natural and engineered inteins have failed previously to function when being flanked by proline residue at the 1 or +2 positions, which limits general uses of inteins.

In this study, different engineered inteins were tested.

We found that engineered Ssp DnaX mini-intein and split inteins could carry out protein splicing with proline at the +2 positions or at both 1 and +2 positions.

Under in vivo conditions in E.

coli cells, the mini-intein, S1 split intein, and S11 split intein spliced efficiently, whereas the S0 split intein did not splice with proline at both 1 and +2 positions.

The S1 and S11 split inteins also trans-spliced efficiently in vitro with proline at the +2 positions or at both 1 and +2 positions, but the S0 split intein trans-spliced inefficiently with proline at the +2 position and did not trans-splice with proline at both 1 and +2 positions.

These findings contribute significantly to the toolbox of intein-based technologies by allowing the use of inteins in proteins having proline at the splicing point.