Enhanced production of fibrinolytic enzyme by a new xanthomonas oryzae IND3 using low-cost culture medium by response surface methodology
Joint Authors
Arasu, Mariadhas Valan
Rajan, R. Anantha
al-Dhabi, N. A.
Vijayaraghavan, Ponnuswamy
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 2 (28 Feb. 2019), pp.217-224, 8 p.
Publisher
Publication Date
2019-02-28
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
- Methodology
- Enzymes
- Biological response modifiers
- Fibrinolysis
- Xanthomonas oryzae
- Response surfaces(Statistics)
Abstract EN
Cardiovascular diseases (CVDs) cause high mortality throughout the world.
Existing fibrinolytic agents are highly expensive and have many side effects.
Microbial fibrinolytic enzymes are very much considered as novel therapeutic candidate for the treatment of CVDs.
Reports on fibrinolytic enzyme from Xanthomonas sp.
is lacking.
This study reports fibrinolytic enzymes from Xanthomonas oryzae IND3 as it shows hyperactivity on fibrin-agarose plates.
This organism utilized various agro-industrial wastes for enzymes production.
Among all, cow dung enhanced more enzyme production, hence it was used as the low-cost substrate for statistical optimization of fibrinolytic protease in Solid state fermentation.
Response surface methodology was employed to optimize the factors and enhanced yield by 4-fold.
The interactions among the variables, viz, sucrose, yeast extract, and pH of the medium were investigated using Central Composite Design (CCD).
The predicted fibrinolytic enzyme activity was 2340 U/g, and the observed fibrinolytic enzyme activity was 2294 ± 12.8 U/g.
The fibrinolytic enzyme degraded blood clot in vitro completely.
This study is the first report on statistical optimization of fibrinolytic enzyme productioninSSFfromXanthomonas sp.Thecrudeextracthasimmense activityonproteinaceous wastes.
The production of fibrinolytic protease using the low-cost substrate could reduce the production cost of enzyme.
American Psychological Association (APA)
Vijayaraghavan, Ponnuswamy& Arasu, Mariadhas Valan& Rajan, R. Anantha& al-Dhabi, N. A.. 2019. Enhanced production of fibrinolytic enzyme by a new xanthomonas oryzae IND3 using low-cost culture medium by response surface methodology. Saudi Journal of Biological Sciences،Vol. 26, no. 2, pp.217-224.
https://search.emarefa.net/detail/BIM-891549
Modern Language Association (MLA)
Vijayaraghavan, Ponnuswamy…[et al.]. Enhanced production of fibrinolytic enzyme by a new xanthomonas oryzae IND3 using low-cost culture medium by response surface methodology. Saudi Journal of Biological Sciences Vol. 26, no. 2 (Feb. 2019), pp.217-224.
https://search.emarefa.net/detail/BIM-891549
American Medical Association (AMA)
Vijayaraghavan, Ponnuswamy& Arasu, Mariadhas Valan& Rajan, R. Anantha& al-Dhabi, N. A.. Enhanced production of fibrinolytic enzyme by a new xanthomonas oryzae IND3 using low-cost culture medium by response surface methodology. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 2, pp.217-224.
https://search.emarefa.net/detail/BIM-891549
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 223-224
Record ID
BIM-891549