Comparison of PCR-RFLP and PFGE for determining the clonality of brucella isolates from human and livestock specimens
Joint Authors
Hashimi, Sayyid Hamid
Arabestani, Muhammad Rida
Bahmani, Nisrin
Mohajerie, Parviz
Karami, Manoochehr
Mirnejad, Rida
Alikhani, Muhammad Yusuf
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 2 (28 Feb. 2019), pp.256-262, 7 p.
Publisher
Publication Date
2019-02-28
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Brucellosis is an important zoonotic disease caused by different species of genus Brucella that are pathogenic for humans and a variety of animals.
Accurate detection of Brucella spp.
infection is important for control of disease.
The aim of this study was to comparison of molecular genotyping of Brucella strains by Pulsed-field gel electrophoresis (PFGE) and polymerase chain reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) techniques.
Twenty- seven Brucella spp.
were isolated from human and animal samples.
The isolates identified by conventional microbiological methods and confirmed using PCR for amplification of omp2a gene.
Molecular typing of Brucella strains carried out by PCR-RFLP after PstI and PFGE of chromosomal DNA after XbaI enzyme digestion.
The omp2a gene PCR Products with different patterns of PCR-RFLP were sequenced.
The omp2a gene amplification of all human and animal Brucella isolates were positive for 1100 bp fragment.
By PCR-RFLP analysis two genotypes/patterns for human isolates and four genotypes for animal isolates were obtained.
In PFGE analysis totally, 7 common clones/clusters and 3 single clones were obtained.
The results of this study showed the PFGE method is the more reliable and useful assay for molecular typing of Brucella strains and is more preferred to PCR-RFLP in determination of genetic similarity among human and animal Brucella isolates.
The presented data showed PCR-RFLP analysis was not able to differentiate between B.
melitensis biovars and vaccine strain
American Psychological Association (APA)
Bahmani, Nisrin& Mirnejad, Rida& Arabestani, Muhammad Rida& Mohajerie, Parviz& Hashimi, Sayyid Hamid& Karami, Manoochehr…[et al.]. 2019. Comparison of PCR-RFLP and PFGE for determining the clonality of brucella isolates from human and livestock specimens. Saudi Journal of Biological Sciences،Vol. 26, no. 2, pp.256-262.
https://search.emarefa.net/detail/BIM-891613
Modern Language Association (MLA)
Bahmani, Nisrin…[et al.]. Comparison of PCR-RFLP and PFGE for determining the clonality of brucella isolates from human and livestock specimens. Saudi Journal of Biological Sciences Vol. 26, no. 2 (Feb. 2019), pp.256-262.
https://search.emarefa.net/detail/BIM-891613
American Medical Association (AMA)
Bahmani, Nisrin& Mirnejad, Rida& Arabestani, Muhammad Rida& Mohajerie, Parviz& Hashimi, Sayyid Hamid& Karami, Manoochehr…[et al.]. Comparison of PCR-RFLP and PFGE for determining the clonality of brucella isolates from human and livestock specimens. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 2, pp.256-262.
https://search.emarefa.net/detail/BIM-891613
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 261-262
Record ID
BIM-891613