GC-MS analysis of fungal filtrate crude extracts produced by two species of Aspergillus
Joint Authors
al-Hasan, Dhurgham A.
Dawud, Ahmad Q.
al-Fartusi, Hind H.
Source
Journal of College of Education for Pure Sciences
Issue
Vol. 9, Issue 1 (31 Mar. 2019), pp.271-283, 13 p.
Publisher
University of Thi-Qar College of Education for Pure Sciences
Publication Date
2019-03-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The species of the genus Aspergillus have an ability to produce secondary metabolites, however, not all filamentous fungi can form the metabolites.
The current study showed that two species of the genus grew at 27 °C on potato dextrose agar (PDA).
Aspergillus terreus appeared brown colonies on the PDA but A.
niger grew as black colonies.
Potato dextrose broth (PDB) used to be a fermenting medium by which the fungal filtrate of both fungi separated from the mycelia.
Residues of the extracted filtrates gave the blackish brown crude extracts.
The extract weights recorded 300 mg/L obtained from A.
niger, and 200 mg/L of A.
terreus.
GC-MS analysis revealed the extract of A.
niger consists of three compounds are {[5-methyl-2-phenylindolizine],[thiocarbamicacid,N,Ndimethyl, S-1,3-diphenyl-2-butenylester], and [22-beta.-acetoxy-3.beta.,16.alpha.- dihydroxy13,28 epoxyolean-2]}.
A.
terreus extract possesses four compounds are {[2.2] paracyclophane, [5-methyl-2-phenylindolizine],[2H-1-benzopyran-6-ol,3,4- dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltride)] , and [dI-.alpha.- tocopherol]}.
300 μL taken from 10 mg/ml of A.
niger crude extract led to hemolysis of red blood cells while 800 μL of the same concentration from A.
terreus extract gave the hemolysis.
The present study concluded that 5-methyl-2- phenylindolizine was detected a similar compound in crude extracts of both A.niger and A.terreus with the same retention time.
It may be attributed to the presenting same gene within the genome of both fungi led to producing 5-methyl-2- phenylindolizine.
American Psychological Association (APA)
al-Hasan, Dhurgham A.& Dawud, Ahmad Q.& al-Fartusi, Hind H.. 2019. GC-MS analysis of fungal filtrate crude extracts produced by two species of Aspergillus. Journal of College of Education for Pure Sciences،Vol. 9, no. 1, pp.271-283.
https://search.emarefa.net/detail/BIM-891731
Modern Language Association (MLA)
al-Hasan, Dhurgham A.…[et al.]. GC-MS analysis of fungal filtrate crude extracts produced by two species of Aspergillus. Journal of College of Education for Pure Sciences Vol. 9, no. 1 (Mar. 2019), pp.271-283.
https://search.emarefa.net/detail/BIM-891731
American Medical Association (AMA)
al-Hasan, Dhurgham A.& Dawud, Ahmad Q.& al-Fartusi, Hind H.. GC-MS analysis of fungal filtrate crude extracts produced by two species of Aspergillus. Journal of College of Education for Pure Sciences. 2019. Vol. 9, no. 1, pp.271-283.
https://search.emarefa.net/detail/BIM-891731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 282-283
Record ID
BIM-891731