Study of killing effects of neutron and UV light on bacteriophage in a dry state by chemiluminescence

Abstract EN

This work is concerned to the study of the effects of ionizing radiation including (beta, neutron and ultraviolet rays) on the high virulence strains of Escherichia coli (O157: H7 and O26),which are known for their high toxicity and high susceptibility to free radicals .

Besidesthe solution of white blood cells containing the microphage were prepared , microphages have the ability to produce free radicals during the process of killing and infection of bacteria.

Increasing or decreasing the effectiveness of bacteria as well as white blood cells are done by reading the chemilumenscence,Which produced by free radical, generate from the oxidation of a certain chemilumenscence reagent, named: Lucigenine, to increase leukocyte toxicity from bacterial toxicity, low-dose of ionized radiation have been used.

The luminescence of individual , mixed microphages and bacteria have been recorded, through chemical glare, ie, that the pellets shine larger than the bacteria.

The readings were taken individually out-of-body state (invitro).The readings for whole blood were taken, which are simulated to the readings inside the body (invivo).