Cloning and expression of a lipase gene from pseudomonas aeruginosa into E.coli
Other Title(s)
الكلونة و التعبير لجين اللايبيز من بكتريا pseudomonas aeruginosa في E.coli
Joint Authors
Awdah, Jasim Muhammad
Khalifah, Mustafa Ibrahim
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 50, Issue 3 (30 Jun. 2019), pp.768-775, 8 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Fifteen local isolates of Pseudomonas were obtained from several sources such as soil, water and some high-fat foods (Meat, olives, coconuts, etc.).
The ability of isolates to produce lipase was measured by the size of clear zone on Tween 20 solid medium and by measuring the enzymatic activity and specific activity.
Isolate M3 (as named in this study) was found to be the most efficient for the production of the lipase with enzymatic activity reached 56.6 U/ml and specific activity of 305.94 U/mg.
This isolate was identified through genetic analysis of the 16S rRNA gene.
and it was shown that the isolate M3 belongs to Pseudomonas aeruginosa with 99% similarity.
The DNA of isolate M3 was extracted and lipase gene was amplified through PCR technique, then purified and cloned into E.coli DH5α cells first using pTG19-T plasmid, and expressed in E.coli Bl21 with expression vector pet-28a.
The activity of lipase from transformed E.coli Bl21 was 196.6 U/ml and the specific activity 618.2 U/mg.
American Psychological Association (APA)
Awdah, Jasim Muhammad& Khalifah, Mustafa Ibrahim. 2019. Cloning and expression of a lipase gene from pseudomonas aeruginosa into E.coli. The Iraqi Journal of Agricultural Science،Vol. 50, no. 3, pp.768-775.
https://search.emarefa.net/detail/BIM-892799
Modern Language Association (MLA)
Awdah, Jasim Muhammad& Khalifah, Mustafa Ibrahim. Cloning and expression of a lipase gene from pseudomonas aeruginosa into E.coli. The Iraqi Journal of Agricultural Science Vol. 50, no. 3 (2019), pp.768-775.
https://search.emarefa.net/detail/BIM-892799
American Medical Association (AMA)
Awdah, Jasim Muhammad& Khalifah, Mustafa Ibrahim. Cloning and expression of a lipase gene from pseudomonas aeruginosa into E.coli. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 3, pp.768-775.
https://search.emarefa.net/detail/BIM-892799
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 774-775
Record ID
BIM-892799