Azadirachta indica leaves mediated green synthesized copper oxide nanoparticles induce apoptosis through activation of TNF-a and caspases signaling pathway against cancer cells
Joint Authors
Roy, Somenath
Chattopadhyay, Dipankar
Dey, Aditi
Manna, Subhankar
Mondal, Dipankar
Raj, Anupam
Das, Subhajit
Bag, Braja Gopal
Chattopadhyay, Sourav
Source
Journal of Saudi Chemical Society
Issue
Vol. 23, Issue 2 (28 Feb. 2019), pp.222-238, 17 p.
Publisher
Publication Date
2019-02-28
Country of Publication
Saudi Arabia
No. of Pages
17
Main Subjects
Topics
- Cancer
- Chemical synthesis
- Immunotherapy
- Antineoplastic drugs
- Copper oxide
- Nanoparticles
- Azadirachta indica
- Apoptosis
Abstract EN
Green nanotechnology elucidates highly prioritized anticancer activity.
We synthesized Copper oxide nanoparticles (CuONPs) using leaves of Azadirachta indica (A.
indica) plants and studied the molecular mechanism of cancer cell apoptosis.
After their synthesis, with the help of expository tools like Fourier transform infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Dynamic light scattering (DLS) and surface zeta potential we confirmed the successful synthesis of CuONPs.
Here, crystalline structure of green synthesized CuONPs of 36 ±8 nm size and spherical shape was able to kill MCF-7 and Hela cells, estimated by MTT assay.
Successful internalization of Cu+2 ions inside the cell was estimated by the atomic absorption study.
Cellular uptake of Cu+2 ions inflicted significant Reactive Oxygen Species (ROS) generation inside the cancer cells, thereby leading to DNA fragmentation as observed by DAPI staining.
In in vivo model, CuONPs reduced the breast tumor volume in Balb/C mice and increased the mean survival time through the alteration of pro-inflammatory cytokines level.
In case of both in vivo and in vitro models, CuONPs altered the pro-inflammatory cytokine level and pro-apoptotic protein expressions.
In future, green synthesized CuONPs might be beneficial for its application as an anticancer drug in in vivo (mice model) and in vitro, though further study is needed on its toxicity
American Psychological Association (APA)
Dey, Aditi& Manna, Subhankar& Chattopadhyay, Sourav& Mondal, Dipankar& Chattopadhyay, Dipankar& Raj, Anupam…[et al.]. 2019. Azadirachta indica leaves mediated green synthesized copper oxide nanoparticles induce apoptosis through activation of TNF-a and caspases signaling pathway against cancer cells. Journal of Saudi Chemical Society،Vol. 23, no. 2, pp.222-238.
https://search.emarefa.net/detail/BIM-892808
Modern Language Association (MLA)
Dey, Aditi…[et al.]. Azadirachta indica leaves mediated green synthesized copper oxide nanoparticles induce apoptosis through activation of TNF-a and caspases signaling pathway against cancer cells. Journal of Saudi Chemical Society Vol. 23, no. 2 (Feb. 2019), pp.222-238.
https://search.emarefa.net/detail/BIM-892808
American Medical Association (AMA)
Dey, Aditi& Manna, Subhankar& Chattopadhyay, Sourav& Mondal, Dipankar& Chattopadhyay, Dipankar& Raj, Anupam…[et al.]. Azadirachta indica leaves mediated green synthesized copper oxide nanoparticles induce apoptosis through activation of TNF-a and caspases signaling pathway against cancer cells. Journal of Saudi Chemical Society. 2019. Vol. 23, no. 2, pp.222-238.
https://search.emarefa.net/detail/BIM-892808
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 236-238
Record ID
BIM-892808