Degradation efficiency of phenolic compounds using immobilized peroxidase purified from soybean
Other Title(s)
كفاءة التفكيك للمركبات الفينولية باستعمال البيروكسيديز المقيد و المنقى من فول الصويا
Joint Authors
Jasim, Aishah Muhammad
Aziz, Ghazi Munim
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 50, Issue 3 (30 Jun. 2019), pp.928-935, 8 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
This study was aimed on purification, immobilization and application of peroxidase enzyme from whole plant of soy bean for the decolorization of textile dyes and degradation of some phenolic compounds.
The purification of peroxidase was in two steps include: concentration by sucrose and gel filtration by using sephadex G-150.
The purification fold was 4.46 and 2.09 with an enzyme yield of 22.34% and 25.55% for peak 1 and peak 2 respectively.
The purified peroxidase enzyme was immobilized by two methods include: covalent linkage immobilization by gluteraldehyde activated chitosan and entrapment by sodium alginate and agarose gel, the immobilization ratio reached to 58.07%, 50% and 11.75% respectively.
Crude, purified and immobilized peroxidase were studied with textile dyes (red, blue, yellow and black) after 24 hr, the maximum removal efficiency was with immobilized peroxidase reached to 13.25, 35.12, 14.37 and 26.92 % respectively.
Immobilized peroxidase on chitosan was able to degrade some phenolic compounds (tannic acid, naphthalene and gallic acid) during 2 hr, and the degradation efficiency was reached to 95.85%, 79.75% and 33.88% respectively.
American Psychological Association (APA)
Jasim, Aishah Muhammad& Aziz, Ghazi Munim. 2019. Degradation efficiency of phenolic compounds using immobilized peroxidase purified from soybean. The Iraqi Journal of Agricultural Science،Vol. 50, no. 3, pp.928-935.
https://search.emarefa.net/detail/BIM-893018
Modern Language Association (MLA)
Jasim, Aishah Muhammad& Aziz, Ghazi Munim. Degradation efficiency of phenolic compounds using immobilized peroxidase purified from soybean. The Iraqi Journal of Agricultural Science Vol. 50, no. 3 (2019), pp.928-935.
https://search.emarefa.net/detail/BIM-893018
American Medical Association (AMA)
Jasim, Aishah Muhammad& Aziz, Ghazi Munim. Degradation efficiency of phenolic compounds using immobilized peroxidase purified from soybean. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 3, pp.928-935.
https://search.emarefa.net/detail/BIM-893018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 934-935
Record ID
BIM-893018