Serum level and single-nucleotide polymorphisms of toll-like receptor-7 among urinary bladder cancer Iraqi patients

Abstract EN

Background: Toll-like receptor 7 (TLR7), a member of TLR family, plays a pivotal role in pathogenesis of different malignancies.

Among these is urinary bladder cancer (UBQ, which has not been extensively studied.

Therefore, it was aimed to determine TLR7 serum level in UBC patients and evaluate its association with some demographic and clinicopathobgical characteristics.

In addition, four 7W single-nucleotide polymorphisms (SNPs: rs179018, rs179019, rs179020, and rs179021) were investigated to determine their susceptibility role in UBC and inspect SNR's impact on TLR7 leveL Sixty-six UBC Iraqi patients were enrolled in this case-control study.

Two control samples were also involved, 40 urinary tract infection (UTT) patients, and 48 healthy control subjects.

Results: Wale gender, older age, and agarette-smoking are risk factors for UBC.TLR7 level showed a significant decreased median in UBC patients compared to UT1 patients or control (1.4 vs.

8.1 and 95 ng/ml, respectively; p < 0.001).

The decrease was more pronounced in males, age groip >48 years, cigarette-smokers, alcohol non-consumers, dinical stages Ml, and superfiaal tumor, as well as patients with family history of cancer and untreated patients.

Mitomycin C and Bacilli* Calmette-Guerin therapies tended to increase TIR7 level.

Among the four investigated SNPs,only rs179019C allele showed significantly uncorrected increased frequency in UBC males compared to control males (p = 0.038), while among UT1 females, C allele frequency maintained a significantly corrected decreased frequency compared to control females (p= 0005).

Some SNPs influenced serum level of TIR7, but a significant impact was recorded for rs179019 in UT1 females lp= 0006).

Conclusions: Downregulation of TIR7 is suggested to have a role in etiology and pathogenesis of UBQ especially the male, elderly and smoker patients.

Mitomycin C and Bacillus Calmette-Gu£rin may enhance TIR7 production in the blood of UBC patients.

7I/?7SNPs are suggested to influence susceptibility to d eve bp UBC, and their potential in impacting TLR7 serum level is augmented.