Effect of acellular bovine pericardium and urinary bladder submucosa matrixes in reconstruction of ventro-lateral hernias in bucks : molecular evaluation

Abstract EN

The present study aimed to estimate the efficiency of both a cellular bovine pericardium and bovine urinary bladder matrix sheets in the reconstruction of large ventro-lateral hernias in Iraqi bucks by using of molecular evaluation depending on real time-polymerase chain reaction technique to investigate the level of basic-fibroblast growth factor and vascular endothelial growth factor genes during the healing process and reconstruction of the abdominal defects.

Under sedation and local anesthesia, (6cm X 8cm size) of ventro-lateral hernias were induced in 24 of Iraqi bucks.

The animals were divided randomly into two main equal groups.

In bovine pericardium-treatment group, the hernias were treated with onlay implantation of bovine pericardium.

While, the hernias in UBM-treatment group were treated with onlay implantation of urinary bladder matrix, 30 days post-inducing of hernias.

The molecular evaluation along the period of following-up recorded a significant up-regulation of the level of basic-fibroblast growth factor gene specific for presence of fibroblasts, myofibroblasts and collagen deposition in urinary bladder matrix -treatment group in comparison to bovine pericardium -treatment group with significant difference even at the end of the study.

While, a significant up regulation of the levels of angiogenesis classic gene vascular endothelial growth factor were recorded in the bucks of bovine pericardium -treatment group compared to urinary bladder matrix -treatment group.

In conclusion; molecular detection of the level of growth factors in target tissue can be used as an important criterion.