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Evaluation of cross protective efficacy of commercial vaccines against Mannheimia haemolytica in mice
Other Title(s)
تقييم كفاءة الحماية التصالبية للقاحات التجارية ضد جرثومة M. haemolytica في الفئران
Joint Authors
Muhammad, Ansam Khalid
Muhammad, Rua Jasim
Ahmad, Wafa Abd al-Ilah
Abd Allah, Asma Hammudi
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 43, Issue 1 (30 Jun. 2019), pp.165-170, 6 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
Mannheimia haemolytica together with Pasteurella multocida represents as a major bacterial causative agent of cattle, sheep and goats respiratory diseases and its one of the most important causes for economic losses to these animals .Commercially available vaccines were used to prevent infections caused by P.
multocida and M.
haemolytica.
Thus, the aim of the present study was to evaluate the cross protection efficacy of two vaccines to protect mice against M.haemolytica, studying humeral immunity, using Enzyme-Linked Immunosorbent Assay.
Forty five mice were divided into three equal groups, group one and two were inoculated subcutaneously 4μlJOVAPAST® and 1μl of Al-kindy vaccines respectively, while the third group was with 0.5 ml sub cutaneous PBS.
LD50for M.haemolytica was estimated as 2× 106 cfu ml and challenge test was conducted by dropping 0.05 ml 2× 106 cfu ml intranasally after three weeks of immunization for the three groups.
The results of Enzyme-Linked Immunosorbent Assay, showed significant increase of antibody titters at (P<0.01) in (group 1 and 2) after first and second weeks post immunization, in comparison with control group.
Also, the re-isolation of M.haemolytica from lungs tissue of all groups after challenged were positive with significant difference between control and immunized group, control group was 4× 108 cfu ∕ml which was higher than immunized group one and group two,which were 2.5×104 cfu∕ml and 3,5×105 cfu∕ml respectively after 24 hour of vaccine.
In conclusion, the two commercial vaccines showed good cross protection efficacy against M.
haemolytica, but JOVAPAST® vaccine showed higher efficacy than Alkindy vaccine, as that it contain two heterologous killed strains and providing the basis for production a vaccine from the two pathogen of local strains.
American Psychological Association (APA)
Ahmad, Wafa Abd al-Ilah& Abd Allah, Asma Hammudi& Muhammad, Ansam Khalid& Muhammad, Rua Jasim. 2019. Evaluation of cross protective efficacy of commercial vaccines against Mannheimia haemolytica in mice. Iraqi Journal of Veterinary Medicine،Vol. 43, no. 1, pp.165-170.
https://search.emarefa.net/detail/BIM-894271
Modern Language Association (MLA)
Ahmad, Wafa Abd al-Ilah…[et al.]. Evaluation of cross protective efficacy of commercial vaccines against Mannheimia haemolytica in mice. Iraqi Journal of Veterinary Medicine Vol. 43, no. 1 (2019), pp.165-170.
https://search.emarefa.net/detail/BIM-894271
American Medical Association (AMA)
Ahmad, Wafa Abd al-Ilah& Abd Allah, Asma Hammudi& Muhammad, Ansam Khalid& Muhammad, Rua Jasim. Evaluation of cross protective efficacy of commercial vaccines against Mannheimia haemolytica in mice. Iraqi Journal of Veterinary Medicine. 2019. Vol. 43, no. 1, pp.165-170.
https://search.emarefa.net/detail/BIM-894271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 168-169
Record ID
BIM-894271