Molecular and phylogenetic study of Theileria spp isolated from ticks in al-Diwaniyah city, Iraq
Other Title(s)
دراسة جزيئية و نسلية للـ Theileria spp المعزولة من قراد في مدينة الديوانية، العراق
Joint Authors
al-Fatlawi, Munir Abd al-Amir Abd
al-Fatlawi, Marwah Salim Hajil
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 50, Issue 1 (28 Feb. 2019), pp.475-479, 5 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2019-02-28
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
The current protozoal study was intended to explore the presence and the evolutionary history of Theileria spp in ticks that infecte 150 out of 200 cows in Al-Diwaniyah City, Iraq.
For these purposes, 10 ticks were collected from each cow, identified morphologically, and crushed for extract Theileria-based DNA from the tick tissues.
The extracted DNA was examined by polymerase chain reaction (PCR) technique for molecularly identificationusing special primer of 18S rRNAgene.
The PCR-targeted products of the 18S rRNA gene were subjected to sequencing for further confirmation of the diagnosis of the Theileria spp.
The results revealed 2 distinct sequenced protozoa, SP1 and SP2.
After performing database searching and comparing the current 2 isolates to some regional and global species, the results showed that the SP1 is closely related to Theileria lestoquardi, KP342263.1 and the SP2 is closely related to Theileria annulata, MF287951.1.
The study reveals important information about the evolutionary history of these protozoa in Al-Diwaniyah City, Iraq.
American Psychological Association (APA)
al-Fatlawi, Marwah Salim Hajil& al-Fatlawi, Munir Abd al-Amir Abd. 2019. Molecular and phylogenetic study of Theileria spp isolated from ticks in al-Diwaniyah city, Iraq. The Iraqi Journal of Agricultural Science،Vol. 50, no. 1, pp.475-479.
https://search.emarefa.net/detail/BIM-894668
Modern Language Association (MLA)
al-Fatlawi, Marwah Salim Hajil& al-Fatlawi, Munir Abd al-Amir Abd. Molecular and phylogenetic study of Theileria spp isolated from ticks in al-Diwaniyah city, Iraq. The Iraqi Journal of Agricultural Science Vol. 50, no. 1 (2019), pp.475-479.
https://search.emarefa.net/detail/BIM-894668
American Medical Association (AMA)
al-Fatlawi, Marwah Salim Hajil& al-Fatlawi, Munir Abd al-Amir Abd. Molecular and phylogenetic study of Theileria spp isolated from ticks in al-Diwaniyah city, Iraq. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 1, pp.475-479.
https://search.emarefa.net/detail/BIM-894668
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 478-479
Record ID
BIM-894668