Spectrophotometric determination of sulfamethoxazole in pure and in pharmaceutical preparations by diazotization and coupling reaction
Other Title(s)
التقدير الطيفي للسلفاميثوكسازول بحالته النقية و في المستحضرات الصيدلانية بوساطة الازوتة و الاقتران
Joint Authors
al-Hamdani, Nasim M.
Abd al-Qadir, Asma N.
Muhammad, Salim A.
Source
Issue
Vol. 28, Issue 3 (30 Jun. 2019), pp.15-26, 12 p.
Publisher
University of Mosul College of Science
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract EN
A highly sensitive, simple and accurate spectrophotometric method has been developed for quantitative determination of sulfamethoxazole(SMX) in both pure form and pharmaceutical preparations.
In this method SMX is diazotized with equimolar of sodium nitrite(NaNO2) in acid medium of hydrochloric acid to form diazonium ion , which is reacted with 2,4,6-trihydroxybenzoic acid in alkaline medium of NaOH to form a yellow water soluble azo dye that has absorption maximum at 416 nm versus reagent blank.
Beer's law is obeyed over the concentration range 0.2-16 μg.
ml-1 with an excellent determination coefficient (r2 = 0.9996) and molar absorptivity 1.84 × 104 l.mol-1.cm-1.
The recoveries are obtained in the range of 97.8 - 99.8% and the relative standard deviation is better than ± 0.23 % .
The stoichiometry of the resulting azo dye has been also worked out and it is found to be 1:1 SM X : 2,4,6-trihydroxybenzoic acid.
This method has been applied successfully for the determination of SMX in pharmaceutical preparations (tablets and oral suspension).
American Psychological Association (APA)
Muhammad, Salim A.& al-Hamdani, Nasim M.& Abd al-Qadir, Asma N.. 2019. Spectrophotometric determination of sulfamethoxazole in pure and in pharmaceutical preparations by diazotization and coupling reaction. Rafidain Journal of Science،Vol. 28, no. 3, pp.15-26.
https://search.emarefa.net/detail/BIM-894752
Modern Language Association (MLA)
Muhammad, Salim A.…[et al.]. Spectrophotometric determination of sulfamethoxazole in pure and in pharmaceutical preparations by diazotization and coupling reaction. Rafidain Journal of Science Vol. 28, no. 3 (2019), pp.15-26.
https://search.emarefa.net/detail/BIM-894752
American Medical Association (AMA)
Muhammad, Salim A.& al-Hamdani, Nasim M.& Abd al-Qadir, Asma N.. Spectrophotometric determination of sulfamethoxazole in pure and in pharmaceutical preparations by diazotization and coupling reaction. Rafidain Journal of Science. 2019. Vol. 28, no. 3, pp.15-26.
https://search.emarefa.net/detail/BIM-894752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 24-25
Record ID
BIM-894752