Spectrophotometric determination of paracetamol using diazotization coupling reaction

Other Title(s)

التقدير الطيفي للباراسيتامول باستخدام تفاعل الأزوتة و الاقتران

Author

Dhannun, Inas S.

Source

Rafidain Journal of Science

Publisher

University of Mosul College of Science

Publication Date

2019-04-30

Country of Publication

Iraq

No. of Pages

8

Main Subjects

Chemistry

Topics

• Measurement
• Spectrometers
• Chemical reactions
• Acetaminophen
• Histidine

English Abstract

An accurate, simple, and sensitive indirect spectrophotometric method which proposed and developed for the determination of paracetamol in different pharmaceutical preparations.

The proposed method was based on acid hydrolysis of PAR to produced p-aminophenol (PAP), PAP was diazotization with nitrite ion to form the corresponding diazonium salt, followed by coupling with histidine reagent in alkaline medium to produced azo dye that showed maximum absorbance at 430 nm.

Beer's law was obeyed in the concentration rage of 10-500 μg/20 ml (i.e.

0.5-25 ppm).

The molar absorpitivity and Sandell's sensitivity of the dye were.

1.118×104 l.mol-1.cm-1and 0.0135 μg.cm-2 respectively.

The method successfully has been applied for the determination of PAR in pure form,and its pharmaceutical preparations (tablets, syrup and injection).

Data Type

Conference Papers

Record ID

BIM-894943

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