A creative indirect spectrophotometric determination of naproxen

Abstract EN

A New, sensitive spectrophotometric method for determination of Naproxen (Nap) has been proposed.

The method is based on a modification of Naproxen into a hydroxy analog (mNap).

The modified compound is coupled with the diazotized p-aminobenzoic acid (PABA) in alkaline medium, the resulting orange azo dye exhibit a maximum absorption at 500 nm.

Beer's law is obeyed over the concentration range from 10 to 650 µg/20 ml, (i.e.

0.5-32.5 µg/ ml) with a good sensitivity (molar absorptivity 2.14x104 l.mol-1 .cm -1), good precision (RSD% better than ±1.93%) and high accuracy (relative error % better than 0.43%), Sandell's sensitivity index is 0.0107µg.cm-2 and the limit of detection (LOD) is 0.024 µg/ml and the limit of quantitation (LOQ) is 0.082 µg/ml.

The method has been applied successfully for determination of Naproxen in dosage forms after extraction of the active ingredient.