Serological survey of anaplasma marginale (rickettsia)‎ antibodies in animals by major urface protein 5 competitive inhibition enzyme-linked immunosorbent assay

Abstract EN

The prevalence of bovine Anaplasmosis caused by Anaplasma marginale was studied in 360 cattle and buffaloes maintained at small holder's farmers at Matrouh governorate between months of June 2006 to July 2007.

The incidence of this parasite by using microscopical examination was found to be 74 (37 %) and 95 (59.3 %) of the examined cattle and buffaloes, respectively.

Serologically using rMSP5-cELISA, the incidence of antibodies against Anaplasma marginale was in 134 (67 %) and 125 (78.1 %) of cattle and buffaloes, respectively.

The naturally diseased cases subjected to chemotherapeutic treatment using L.

A oxytetracycline (200 mg / kg b.

wt) showed rapid recovery with an undetectable parasitemia as measured by 28 days post-treatment.

In conclusion, Anaplasmosis is a prevalent disease of bovine farms in the examined area.

Additionally, the ability of the rMSP5-cELISA to accurately detect individually infected animals will facilitate epidemiologic investigations, in areas where the Anaplasmosis expanding through purchasing of infected animals and getting access into disease-free regions.