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Enhanced butanol production by optimization of medium parameters using clostridium acetobutylicum YM1
Joint Authors
Abd al-Shahin, Peyman
Yusuf, Wan Mukhtar Wan
al-Shurgani, Najib Kayid Nasir
Abd al-Hamid, Aidil
Shakur, Hafizah
Kalil, Mohd Sahaid
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 7 (30 Nov. 2018), pp.1308-1321, 14 p.
Publisher
Publication Date
2018-11-30
Country of Publication
Saudi Arabia
No. of Pages
14
Main Subjects
Topics
Abstract EN
A new isolate of the solvent-producing Clostridium acetobutylicum YM1 was used to produce butanol in batch culture fermentation.
The effects of glucose concentration, butyric acid addition and C/N ratio were studied conventionally (one-factor-at-a-time).
Moreover, the interactions between glucose concentration, butyric acid addition and C/N ratio were further investigated to optimize butanol production using response surface methodology (RSM).
A central composite design was applied, and a polynomial regression model with a quadratic term was used to analyze the experimental data using analysis of variance (ANOVA).
ANOVA revealed that the model was highly significant (p< 0.0001) and the effects of the glucose and butyric acid concentrations on butanol production were significant.
The model validation experiment showed 13.82 g/L butanol was produced under optimum conditions.
Scale up fermentation in optimized medium resulted in 17 g/L of butanol and 21.71 g/L of ABE.
The experimental data of scale up in 5 L bioreactor and flask scale were fitted to kinetic mathematical models published in the literature to estimate the kinetic parameters of the fermentation.
The models used gave the best fit for butanol production, biomass and glucose consumption for both flask scale and bioreactor scale up.
American Psychological Association (APA)
al-Shurgani, Najib Kayid Nasir& Shakur, Hafizah& Abd al-Shahin, Peyman& Kalil, Mohd Sahaid& Yusuf, Wan Mukhtar Wan& Abd al-Hamid, Aidil. 2018. Enhanced butanol production by optimization of medium parameters using clostridium acetobutylicum YM1. Saudi Journal of Biological Sciences،Vol. 25, no. 7, pp.1308-1321.
https://search.emarefa.net/detail/BIM-896332
Modern Language Association (MLA)
al-Shurgani, Najib Kayid Nasir…[et al.]. Enhanced butanol production by optimization of medium parameters using clostridium acetobutylicum YM1. Saudi Journal of Biological Sciences Vol. 25, no. 7 (Nov. 2018), pp.1308-1321.
https://search.emarefa.net/detail/BIM-896332
American Medical Association (AMA)
al-Shurgani, Najib Kayid Nasir& Shakur, Hafizah& Abd al-Shahin, Peyman& Kalil, Mohd Sahaid& Yusuf, Wan Mukhtar Wan& Abd al-Hamid, Aidil. Enhanced butanol production by optimization of medium parameters using clostridium acetobutylicum YM1. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 7, pp.1308-1321.
https://search.emarefa.net/detail/BIM-896332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1319-1321
Record ID
BIM-896332