Isolation of enterococcus faecalis from tilapia in lake temsah in ismailia governorate

Abstract EN

This study was applied on 100 naturally diseased Oreoc-hromis niloticus and Tilapia zilli (50 fish each) collected from Lake Temsah in Ismailia governorate to investigate the most prevalent bacterial pathogen.

Clinical signs and postmortum findings of the diseased fishes were done, samples were taken from fins, tails and skin ulcer, liver, kidney, spleen, brain and mouth lips for bacteriological examination which indicated the presence of different bacterial species including Enterococcus faecalis.

The percentage of infection in Nile tilapia with Ent.

faecalis was 23.76 %.

The percentage of infection in Tilapia zilli with Ent.

faecalis was 31.90 %.

Antibiogram test of bacterial isolates recovered from naturally infected Nile tilapia and Tilapia zilli revealed that Amoxicillin (Ax10) and Penicillin (p10) were the drug of choice against Ent.

faecalis.

The results of Polymerase Chain Reaction (PCR) showed appearance of specific bands of Enterococcus spp.

in the kidneys of both tilapias and the brain of Nile tilapia which were identified by multiple sequence alignments of published 16S rRNA sequences and gave a specific band at 733 basepair (bP).